We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to FAQs and tips
  • RNA-seq FAQs
  • RNA-seq tips
  • Positive and negative controls in scRNA-seq
  • DNA-seq FAQs
  • ChIP-seq FAQs
  • Indexing FAQs
  • TCR-seq methods: Q&A
  • STORM-seq Q&A
  • Pushing the limits Q&A
  • Liver metabolic function Q&A
  • Neural multiomics Q&A
Liver metabolic webinar Watch the webinar
Home › Learning centers › Next-generation sequencing › FAQs and tips › Liver metabolic function Q&A

Next-generation sequencing

  • Product line overview
  • Technical notes
    • DNA-seq
      • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
      • Next-gen WGA method for CNV and SNV detection from single cells
      • Low-volume DNA shearing for ThruPLEX library prep
      • Low-input whole-exome sequencing
      • Cell-free nucleic acid sequencing
      • DNA-seq from FFPE samples
      • ThruPLEX HV data sheet
      • Improvements to ThruPLEX HV
      • ThruPLEX HV outperforms NEBNext Ultra II
      • Comparing ThruPLEX HV PLUS to Kapa and NEBNext
      • Low cell number ChIP-seq using ThruPLEX DNA-Seq
      • Accurate detection of low-frequency variants using molecular tags
    • Immune Profiling
      • Efficient and sensitive profiling of human B-cell receptor repertoire
      • TCRv2 kit validated for rhesus macaque samples
      • TCR repertoire profiling from mouse samples (bulk)
      • BCR repertoire profiling from mouse samples (bulk)
      • Improved TCR repertoire profiling from human samples (bulk)
      • TCR repertoire profiling from human samples (single cells)
      • BCR repertoire profiling from human samples (bulk)
    • RNA-seq
      • All-in-one cDNA synthesis and library prep from single cells
      • Automation-friendly, all-in-one cDNA synthesis and library prep
      • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
      • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
      • Full-length mRNA-seq for target capture
      • Stranded libraries from single cells
      • Stranded libraries from picogram-input total RNA (v3)
      • Stranded libraries from 100 pg-100 ng total RNA
      • Stranded libraries from 100 ng - 1 ug total RNA
      • Stranded libraries from FFPE inputs (v2)
      • Nonstranded libraries from FFPE inputs
      • Singular and Takara Bio library prep
    • Epigenetic sequencing
      • ChIP-seq libraries for transcription factor analysis
      • ChIP-seq libraries from ssDNA
      • Full-length small RNA libraries
      • Methylated DNA-seq with MBD2
    • Reproductive health technologies
      • Embgenix PGT-A (CE-IVD & RUO)
  • Featured kits
  • Technology and application overviews
    • SMART technology
    • Single-cell mRNA-seq
    • Total RNA-seq
    • Biomarker discovery with RNA-seq
    • SMART-Seq PLUS solutions
    • Whole genome amplification from single cells
    • Sequencing depth for ThruPLEX Tag-seq
  • FAQs and tips
    • RNA-seq FAQs
    • RNA-seq tips
    • Positive and negative controls in scRNA-seq
    • DNA-seq FAQs
    • ChIP-seq FAQs
    • Indexing FAQs
    • TCR-seq methods: Q&A
    • STORM-seq Q&A
    • Pushing the limits Q&A
    • Liver metabolic function Q&A
    • Neural multiomics Q&A
  • DNA-seq protocols
    • Using UMIs with ThruPLEX Tag-Seq HV
    • Exome capture with Illumina Nextera Rapid Capture
    • Targeted capture with Roche NimbleGen SeqCap EZ
    • Targeted capture with IDT xGen panels
    • Targeted capture with Agilent SureSelectXT
    • Targeted capture with Agilent SureSelectXT2
    • Targeted capture with Agilent SureSelectQXT
    • Plasma preparation for ThruPLEX Plasma-Seq workflow
  • Bioinformatics resources
    • Cogent NGS Analysis Pipeline
      • Cogent NGS Analysis Pipeline notices
    • Cogent NGS Discovery Software
      • Cogent NGS Discovery Software notices
    • Cogent NGS Immune Profiler
      • Cogent NGS Immune Profiler Software notices
      • Cogent NGS Immune Profiler Software videos
    • Cogent NGS Immune Viewer
    • Embgenix Analysis Software
    • ICELL8 scTCR Analyzer
    • SMARTer Human scTCR Demultiplexer
    • SMART-Seq DE3 Demultiplexer
  • Newsletters
    • 2021 H1 NGS newsletter
    • 2020 NGS newsletter
  • Webinars
    • Harnessing the power of full-length transcriptome analysis for biomarker discoveries
    • SMART-Seq Pro kits for biomarker detection
    • Takara Bio Single-Cell Workshop, Spring 2021
    • Capturing biological complexity by high-resolution single-cell genomics
    • Sensitivity and scale for neuron multiomics
    • Taking single-cell RNA-seq by STORM
    • Pushing the limits of sensitivity for single-cell applications
    • Liver metabolic function, dissecting one cell at a time
    • Single-Cell Workshop at 2020 NextGen Omics Series UK
    • TCR-seq methods: when to use which
    • Immunogenomics to accelerate immunotherapy
    • Total RNA sequencing of liquid biopsies
    • MeD-Seq, a novel method to detect DNA methylation
    • Automating full-length single-cell RNA-seq libraries
    • Single-cell DNA-seq
    • Single-cell whole transcriptome analysis
  • Citations
    • Epigenetics citations
    • Microbiome citations
    • PicoPLEX citations
    • SMARTer RNA-seq citations
    • ThruPLEX DNA-seq citations
    • ThruPLEX Plasma-seq citations
  • Posters
New products
Need help?
Contact Sales
Liver metabolic webinar Watch the webinar

Liver metabolic function, dissecting one cell at a time Q&A

Single-cell RNA-seq can help elucidate the role of pathogenic cell populations in the development and progression of chronic diseases. 

In this webinar Dr. Martinez-Jimenez and Dr. Deligiannis present their findings on the practical application of snRNA-seq2, a robust and reliable method for the analysis of the nuclear transcriptome from frozen tissues developed by customizing the Takara Bio SMART-Seq Single Cell Kit . The cost-efficient miniaturization of snRNA-seq2 using liquid handling robots (i.e., the mosquito HV genomics system, SPT Labtech) is also discussed. 

Continue reading for insights from the Q&A session or register to watch the on-demand webinar.

How can we gain access to the scripts for snRNA-seq2?

The Jupyter Notebook code for analysis is available for download here and in Zenodo. The data is now publicly available here and here.

How can we gain access to the scripts for the SMART-Seq Single Cell Kit on mosquito HV?

To receive the mosquito scripts for cDNA synthesis and bead clean-ups, please get in touch with the SPT Labtech Field Applications Team.

If working with multiple cells per well, which kit would you recommend?

In this case, we would recommend the the SMART-Seq v4 Kit.  

The SMART-Seq v4 Kit (SSv4) and the SMART-Seq v4 PLUS Kit (SSv4 PLUS) are the fourth generation of our SMARTer ultra-low solutions and our most sensitive kits for ultra-low inputs. These kits use oligo(dT) priming to generate high-quality, full-length cDNA directly from multiple intact cells (up to 1,000 cells) or 10 pg–10 ng of total RNA.

Can you distinguish between 4n bi- and 4n mono-nucleated cells with the snRNA-seq methodology?

With the transcriptional profile alone, it is difficult to distinguish between 2n nuclei from mononucleated hepatocytes and 2n nuclei from tetraploid binucleated hepatocytes (2n x 2).

Technologies such as RNAScope (in situ hybridization assay for detection of target RNA within intact cells) can be used to distinguish this hepatocyte ploidy. Spatial transcriptomics approaches in the future will be very useful to address these questions genome-wide.

Have you explored differences in splicing variants between polyploid hepatocytes?

Taking advantage of the full-length transcript information that snRNA-seq2 offers, we are now investigating the potential splicing variant differences between hepatocyte nuclei.

Has this strategy been used with other tissues?

In our lab we have used snRNA-seq2 method with liver, gut, brain and muscle tissue.

What was the ratio of volume of lysis buffer/volume of tissue sample used in the study?

For the single nuclei isolation, the protocol described by Krishnaswami et al. was followed with several modifications.

In summary, fresh frozen liver tissues (~3 mm3) were homogenized and the final nuclei suspension was used for FACS sorting of 1 nuclei per well containing 0.9 µL final volume of the Takara Bio lysis buffer.

Do you have optimized protocols for extracting nuclei from human tissues (such as lung)?

We have used the nuclei isolation protocol described in snRNA-seq2 with various tissues from mice, including lung and we obtain a clean suspension. Similarly, we have used it for human tissues, liver and bone marrow but not with human lung. We are positive that it would work although it might require additional optimization to increase the purity of the single-nuclei suspension.

Were differences in ploidy status relevant in stellate cells in fibrotic conditions?

We have not explored the differences on stellate cell ploidy status. We focused on hepatocytes in this study.

How does ploidy correlate with oxidative or glycolytic metabolism?

According to analysis via Gene Ontology between 2n and 4n hepatocytes, an enrichment of genes related to lipid and xenobiotic metabolism as well as oxidative stress pathways is oberserved in 4n hepatocytes. Other pathways can be explored and investigated under different treatments and conditions.

When performing snRNA-seq2 method, could you potentially identify nuclei from binuclear polyploid cells using bioinformatical tools?

Starting from single-nuclei suspensions, we can only distinguish DNA content per nuclei using Hoescht and FACs sorting. We found no significant differences in the mRNA levels between 2n nuclei in diploid and tetraploid binucleated cells using RNAscope, and those populations are not able to be distinguished by snRNA-seq2 computationally.

Does the Takara Bio SMART-Seq Single Cell Kit allow deep characterization of nuclei isolated from fresh tissues?

Both fresh and frozen tissues can be used performing the with the snRNA-seq2 using the SMART-Seq Single Cell Kit. When possible, fresh tissue and whole cell approaches might be preferred, depending on the experimental design, dissociation methods, and accessibility to fresh samples.

Is polyploidy related to liver cell regeneration?

There are several recent publications using lineage tracing strategies to investigate the regenerative potential of tetraploid hepatocytes (Chen F, et al., 2020; Sun T, et al. 2020; Matsumoto T, et al., 2020).

Their work and our work conclude that, both 2n and 4n can contribute to liver regeneration, although 4n cells need to divide into 2n first.

Does the snRNA-seq2 method work for low-input/low-quality samples (e.g. single cells collected from laser microdissection)?

Although we have not used this methodology for single cell/nuclei RNAseq from laser microdissection, the addition of lysis buffer 2 is compatible with downstream SMARTer chemistry. As long as a strategy for placing the cell in wells with lysis buffer is in place, the cDNA generation using the presented pipeline should be straight-forward.

Do you use FACS just for sorting 2n and 4n cells, or it is also used for purification purposes?

For our snRNA-seq2 approach, we use FACS sorting to address ploidy levels in our nuclei suspension and sort single nuclei into wells. The FACS sorting step though is not always necessary for downstream RNAseq, as long as the suspension is clean enough. For single-cell RNAseq based on well-plates, FACS sorting or other instruments for dispensing single-cells/nuclei into weeks will be needed.

Do you miss relevant extranuclear transcripts with snRNA-seq2?

Using snRNA-seq2, only the nuclear mRNA is captured, loosing partial information about the mature cytoplasmic mRNAs. The high correlation between nuclear and whole-cell transcriptome shows that the analysis of nuclear transcripts is a robust and reliable approach for cell-type identification.

Do male and female mice show the same percentage of polyploidies in their livers?

We have not investigated gender bias in hepatocyte ploidy. It will certainly be an interesting question to explore.

Is the data on immunohistochemistry available online?

Our BioRxiv manuscript was recently accepted in Nature Communications, and all data will be publicly available shortly.

Are nuclei from a binuclear cell transcriptionally similar?

In young C57Bl6/J, the expression levels of mononucleated and binucleated hepatocytes are conditioned by their position in the hepatic lobule (confirmed with RNAScope). The global expression profiles of 2n nuclei and 4n nuclei is very similar and they are not easily distinguished without previous knowledge on their genome content (e.g. by DAPI or Hoescht).

Could you detect interference of cryopreservation agents like RNAlater or others with your protocol?

We have not tried our pipeline with RNAlater or other cryopreservation methods. We do not know if cryopreservation reagents would have a major impact in the in nuclear transriptome.

How do you avoid DNA contamination?

In our data, we are not able to detect high DNA contamination sequences. In the case of high DNA content, we can follow the manufacturer's suggestion to pre-treat the samples with DNAse I.

What is the best way to improve nuclei quality and integrity after sort?

To acquire clean nuclei suspension, we use an OptiPrep gradient and inspect the quality under a microscope. For further improvements, washing and filtering steps can be used. To maintain integrity, the appropriate nuclei storage buffer needs to be used for each tissue and all manipulations needs to be done with caution regarding shearing forces.

Is there a difference in RNA expression between 2n and 4n in NASH and HCC?

We are now further investigating the changes in the transcriptome of human chronic liver diseases and whether the percentages of 2n to 4n change as well.


SMART-Seq Single Cell Kit resources

SSsc for scRNA-seq

Generate high-quality, full-length RNA-seq libraries from single cells (e.g., PBMCs) and nuclei.

Highest sensitivity for single-cell mRNA-seq

View data on the SMART-Seq Single Cell Kit's superior sensitivity and reproducibility for single-cell and nuclei applications.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • OEM
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us