Study all BCR isotypes to develop anti-HIV antibodies
To improve the design of HIV vaccines or develop the best antiretroviral therapy strategies, it is important to understand HIV antibodies and the humoral immune response.
B-cell receptor (BCR) profiling is essential in gaining this information, since upon HIV infection, B cells are activated and initiate somatic hypermutation to develop BCRs giving rise to antibodies with increased affinity. Previous research (Mouquet et al. 2010) has shown that these mutation events also gave rise to increased breadth and neutralizing potency.
Knowing more about these changes in BCRs is crucial for selecting the best BCR clones for HIV vaccines or anti-HIV antibodies. To acquire this knowledge, a highly sensitive and unbiased BCR profiling approach is required.
Mouquet, H. et al. Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation. Nature 467, 591–595 (2010).
SMART-Seq Human BCR (with UMIs)
- Capture all BCR isotypes
- Pool up to 384 libraries for high-throughput sequencing using compatible UDIs
- Sequence either the full-length V(D)J region or the CDR3 only
- Set up reactions easily with pooled primers and wide input ranges
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