NDiff 227: neural differentiation medium
NDiff 227 medium is a defined, serum-free, N2- and B-27 supplemented medium for neural differentiation of mouse embryonic stem (ES) cells in adherent monoculture (Ying et al. 2003). NDiff 227 can also be supplemented to enable long-term pluripotent stem cell culture. Successive withdrawal of the supplements results in differentiation into the neural lineage.
A complete, serum-free, ready‐to‐use medium for neural differentiation of mouse ES cells
Supplemented with N2 and B-27
Neural differentiation of mouse ES cells in monoculture
Refer to the Data Sheet for additional examples of use
500 ml NDiff 227 medium
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183-186 (2003).
Additional product information
Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.