NDiff 227: neural differentiation medium
NDiff 227 medium is a defined, serum-free, N2- and B-27 supplemented medium for neural differentiation of mouse embryonic stem (ES) cells in adherent monoculture (Ying et al. 2003). NDiff 227 can also be supplemented to enable long-term pluripotent stem cell culture. Successive withdrawal of the supplements results in differentiation into the neural lineage.
A complete, serum-free, ready‐to‐use medium for neural differentiation of mouse ES cells
Supplemented with N2 and B-27
Neural differentiation of mouse ES cells in monoculture
Refer to the Data Sheet for additional examples of use
500 ml NDiff 227 medium
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183-186 (2003).
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