GS2-M medium: maintenance of germline-competent mouse and human ES and iPS cells

GS2‐M is a completely defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.

GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (Silva et al. 2008; Silva et al. 2009).

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Cat. #	Product	Size	Price	License	Quantity	Details
Y40030	GS2-M®	100 mL	$321.00
GS2-M is a proprietary, defined and serum-free cell culture media formulation for the generation of mouse ES and iPS cell lines in the presence of two selective small molecule inhibitors CHIR99021 and PD0325901 ('2i') that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell survival. LIF can also be added to orchestrate the efficient conversion of partial or pre-iPS mouse cells into fully pluripotent iPS cells via Nanog. Long term maintenance of these naïve, 'ground state' mouse pluripotent stem cells is then solely dependent on GS2-M alone. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Mouse embryonic stem cells were cultured for 3 passages in GS2-M and then labelled for Oct3/4 (red) and Nanog (green), indicating that the cells were pluripotent Mouse embryonic stem cells were cultured for 3 passages in GS2-M and then labelled for Oct3/4 (red) and Nanog (green), indicating that the cells were pluripotent. Back The ground-state pluripotency marker Rex1 was analyzed via flow cytometry in a Rex1-GFP transduced mouse embryonic stem cell line The ground-state pluripotency marker Rex1 was analyzed via flow cytometry in a Rex1-GFP transduced mouse embryonic stem cell line. Prior to analysis, cells were cultured in either GS2-M or 10%FBS/GMEM + LIF for 3 passages. Cells grown in GS2-M exhibited superior ground-state maintenance.

Overview

Completely defined, serum-free formulation
Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state
Allows long-term maintenance of germline-competent mouse and human ES and iPS cells

More Information

Applications

Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*
Conversion of human pre-ES or iPS cells into naïve stem cells*
Maintenance of pure ES and iPS cells in a pluripotent state

* in the presence of forskolin and/or LIF.

Components

100 ml medium
100 µl 2i inhibitor supplement

References

Silva, J. et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008).

Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722-37 (2009).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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