GS2-M medium: maintenance of germline-competent mouse and human ES and iPS cells
GS2‐M is a completely defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.
GS2‐M is a completely defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.
GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (Silva et al. 2008; Silva et al. 2009).
Overview
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Completely defined, serum-free formulation
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Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state
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Allows long-term maintenance of germline-competent mouse and human ES and iPS cells
More Information
Applications
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Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*
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Conversion of human pre-ES or iPS cells into naïve stem cells*
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Maintenance of pure ES and iPS cells in a pluripotent state
* in the presence of forskolin and/or LIF.
Components
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100 ml medium
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100 µl 2i inhibitor supplement
References
Silva, J. et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008).
Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722-37 (2009).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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