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MicroRNA first-strand synthesis and miRNA quantitation kits

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

  • First-strand cDNA synthesis from miRNA
  • Quantitative PCR (qPCR) of miRNA

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

  • First-strand cDNA synthesis from miRNA and
  • Quantitative PCR (qPCR) of miRNA

Simple, single-step cDNA synthesis

Unlike other vendors' kits, these microRNA quantitation kits provide a simple, single-step protocol for the first-strand synthesis reaction. Mir-X uses an optimized mix of poly(A) polymerase and SMART MMLV Reverse Transcriptase to synthesize first-strand cDNA from your microRNA-containing RNA sample.

Sensitive qPCR using microRNA-specific primers

cDNA is amplified and quantified by qPCR using your microRNA-specific primer and our TB Green Advantage qPCR Premix. Multiple microRNA species, as well as the mRNA targets of the microRNAs, can be amplified from a single cDNA sample. The system is able to detect microRNAs down to 50 copies.

Highly specific detection of microRNA

To demonstrate the specificity of Mir-X miRNA quantitation, we used a series of 8 highly similar synthetic Let7 miRNA variants. We first spiked each of the Let7 miRNAs into separate samples of yeast polyA+ RNA and generated cDNA using the Mir-X single-tube reaction. We then tested a panel of variant-specific primers with each cDNA sample to determine each primer’s ability to specifically and individually quantify the Let7 subtypes in the cDNA sample. Despite the high degrees of similarity among the variants and the primers, Mir-X qPCR was highly specific in detecting each Let7 variant.

Large format qPCR kits

The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and each kit includes our Mir-X miRNA First-Strand Synthesis Kit and TB Green Advantage qPCR Premix.

 More  Less
Cat. # Product Size Price License Quantity Details
638313 Mir-X™ miRNA First-Strand Synthesis Kit 20 Rxns USD $328.00

License Statement

ID Number  
269 For Research Use Only. Not for diagnostics. Not for use in diagnostic procedures. Product is covered by patents and patent applications owned by Exiqon A/S.

This 20 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio's TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

Back

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

Back

Induction and quantitation of miR-9 miRNA in mouse P19 cells

Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

Back

Trichostatin A treatment alters miRNA expression in mouse ES cells

Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.

Back

638313: Mir-X miRNA First-Strand Synthesis Kit

638313: Mir-X miRNA First-Strand Synthesis Kit
638314 Mir-X™ miRNA qRT-PCR TB Green® Kit 200 Rxns USD $523.00

License Statement

ID Number  
269 For Research Use Only. Not for diagnostics. Not for use in diagnostic procedures. Product is covered by patents and patent applications owned by Exiqon A/S.

This 200 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 600 rxn kit see Cat. # 638316. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

638314: Mir-X miRNA qRT-PCR TB Green Kit

638314: Mir-X miRNA qRT-PCR TB Green Kit

Back

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

Back

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

Back

Induction and quantitation of miR-9 miRNA in mouse P19 cells

Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

Back

Trichostatin A treatment alters miRNA expression in mouse ES cells

Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.
638315 Mir-X™ miRNA First Strand Synthesis Kit 60 Rxns USD $507.00

This 60 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio’s TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 20 rxn kit see Cat. # 638313.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

Back

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

Back

Induction and quantitation of miR-9 miRNA in mouse P19 cells

Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

Back

Trichostatin A treatment alters miRNA expression in mouse ES cells

Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.

Back

638315: Mir-X miRNA First Strand Synthesis Kit

638315: Mir-X miRNA First Strand Synthesis Kit
638316 Mir-X™ miRNA qRT-PCR TB Green® Kit 600 Rxns USD $1038.00

License Statement

ID Number  
269 For Research Use Only. Not for diagnostics. Not for use in diagnostic procedures. Product is covered by patents and patent applications owned by Exiqon A/S.

This 600 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 200 rxn kit see Cat. # 638314.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

638316: Mir-X miRNA qRT-PCR TB Green Kit

638316: Mir-X miRNA qRT-PCR TB Green Kit

Back

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

Back

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

Back

Induction and quantitation of miR-9 miRNA in mouse P19 cells

Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

Back

Trichostatin A treatment alters miRNA expression in mouse ES cells

Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.

Overview

  • Simple, single-step cDNA synthesis reaction
  • Two kits in one: cDNA synthesis and qPCR of microRNA
  • Quantify any microRNA and its target using the same RNA sample
  • Detection of microRNA down to 50 copies
  • High specificity
  • 200 rxn and 600 rxn qPCR kit formats

More Information

Applications

  • cDNA synthesis from microRNA
  • microRNA quantitation by qPCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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  • cDNA synthesis kits
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