Antibiotic selection markers and plasmids

Linear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Linear Hygromycin Marker) or puromycin (Linear Puromycin Marker) resistance. These markers are short, purified linear DNA fragments comprised of the marker gene, an SV40 promoter, and the SV40 polyadenylation signal.

Cotransfection of a linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker or cotransfecting with a circular selection marker. Linear selection markers are cotransfected at a decreased ratio relative to the expression vector (i.e., 20-fold less of the linear marker).

The pTK-Hyg Vector and the pPur Vector may be used to select for stably transformed mammalian cells using hygromycin and puromycin, respectively. When cotransfected with pTRE2 or a pBI vector, pTK-Hyg is especially useful for selecting double-stable Tet-On or Tet-Off Cell Lines. When integrated into the host cell’s genome, pTK-Hyg does not cause unwanted activation of pTRE- and pBI-derived plasmids because the HSV TK promoter lacks an enhancer element.

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Cat. #	Product	Size	Quantity
631626	Linear Puromycin Marker	2 ug	*
Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker. Back 631626: Linear Puromycin Marker
631619	pIRESpuro3 Vector	20 ug	*
pIRESpuro3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV), which permits both the gene of interest and the puromycin selection marker to be translated from a single mRNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 631619: pIRESpuro3 Vector Back Vector maps for IRES selection vectors Vector maps for IRES selection vectors. IRES bicistronic expression vectors permit your gene of interest and a selection marker to be translated from a single mRNA. Nearly 100% of the cells that survive selection also express your protein of interest.
631620	pIREShyg3 Vector	20 ug	*
pIREShyg3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV), which permits both the gene of interest and the hygromycin B selection marker to be translated from a single mRNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Vector maps for IRES selection vectors Vector maps for IRES selection vectors. IRES bicistronic expression vectors permit your gene of interest and a selection marker to be translated from a single mRNA. Nearly 100% of the cells that survive selection also express your protein of interest. Back 631620: pIREShyg3 Vector
631622	pIRESbleo3 Vector	20 ug	*
The pIRESbleo3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) which permits both the gene of interest and the bleomycin selection marker to be translated from a single mRNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Vector maps for IRES selection vectors Vector maps for IRES selection vectors. IRES bicistronic expression vectors permit your gene of interest and a selection marker to be translated from a single mRNA. Nearly 100% of the cells that survive selection also express your protein of interest. Back 631622: pIRESbleo3 Vector
631625	Linear Hygromycin Marker	2 ug	*
Linear Hygromycin Marker is a short ds DNA fragment consisting of the hygromycin resistance gene regulated by the SV40 promoter and ending with the SV40 polyadenylation signal. The 2.1-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 631625: Linear Hygromycin Marker Back Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker.
631750	pTK-Hyg Vector	10 ug	*
Selection vector which confers hygromycin resistance in mammalian cells for the selection of stably transformed cells. This vector does not contain any enhancer elements and therefore is ideal for use in developing double-stable Tet-Off or Tet-On cell lines because it prevents the unwanted activation of pTRE- and pBI-derived plasmids upon cointegration into the host cell's genome. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Map of the pTK-Hyg Vector Map of the pTK-Hyg Vector. This vector encodes a hygromycin resistance marker and is designed for co-transfection with plasmids that lack selection. Back 631750: pTK-Hyg Vector

Overview

Both the linear selection markers and the pTK-Hyg Vector and pPur Vector may be used in cotransfections to establish stable cell lines
Linear selection markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used
Linear selection markers are ideal for use with all Takara Bio's Tet-On and Tet-Off Inducible Expression Systems—screen fewer candidates and find more highly inducible clones

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Applications

Use the Linear Hygromycin Marker or the pTK-Hyg Vector in cotransfections to establish stable cell lines using hygromycin
Use the Linear Puromycin Marker or the pPur Vector in cotransfections to establish stable cell lines using puromycin

Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Antibiotic selection markers and plasmids

Notice to purchaser

Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker

631626: Linear Puromycin Marker

Notice to purchaser

631619: pIRESpuro3 Vector

Vector maps for IRES selection vectors

Notice to purchaser

Vector maps for IRES selection vectors

631620: pIREShyg3 Vector

Notice to purchaser

Vector maps for IRES selection vectors

631622: pIRESbleo3 Vector

Notice to purchaser

631625: Linear Hygromycin Marker

Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker

Notice to purchaser

Map of the pTK-Hyg Vector

631750: pTK-Hyg Vector

Overview

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Applications

Additional product information