The Guide-it In Vitro Transcription Kit contains the necessary reagents for the transcription and cleanup of your sgRNA sequences. It is combined with the Guide-it sgRNA Screening Kit as part of the Guide-it Complete sgRNA screening system which can be used for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies.

The Guide-it Complete sgRNA Screening System includes everything needed for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies, including PCR reagents for amplifying your genomic target and recombinant Cas9 for in vitro analysis of the transcribed sgRNA.

The Guide-it sgRNA Screening Kit contains PCR reagents to amplify genomic DNA from your target cells and recombinant Cas9 for the in vitro screening of your sgRNA to help you pick the best guide sequences before moving to experiments in living cells or animals. It is combined with the Guide-it In VItro Transcription kit as part of the Guide-it Complete sgRNA Screening System.