Guide-it™ Long ssDNA Production System
FACS analysis of AcGFP1 knockin efficiencies at the SEC61B and TUBA1A loci.
PCR analysis of AcGFP1 knockin at the SEC61B locus in clonal populations isolated by FACS.
Toxicity comparison for electroporation of dsDNA or ssDNA.
In vivo expression of AcGFP1 fusion proteins in cells edited using Cas9-sgRNA RNPs and ssDNA HDR templates.