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Cat. # Product Size Price License Quantity Details
T210 Anti-CD3 mAb GMP grade 1 mg/1 mL USD $1530.00

T210, Anti-CD3 mAB GMP grade, is manufactured and tested in accordance with the following guidelines, "Standards for Manufacturing Control and Quality Control, etc. of Investigational Products (Investigational products GMP)" notified by the Ministry of Health and Welfare Japan ("PFSB Notification No. 0709002, July 9, 2008)" (PFSB: Pharmaceutical and Food Safety Bureau).

Anti-CD3 antibodies are commonly used in T lymphocyte proliferation protocols. Among many available clones, "OKT3" (mouse IgG2a) is the most commonly used. This monoclonal anti-CD3 antibody (clone OKT3) is manufactured as a quality-assured product, according to relevant guidelines for Good Manufacturing Practice (GMP). This product can be used for ex vivo cell culture processing in preclinical research and investigational phase studies.

632180 Lenti-X™ 293T Cell Line 1 mL USD $333.00

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with the Lenti-X HTX Packaging System and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108 ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen.

632164 Lenti-X™ Expression System Each USD $1096.00

The Lenti-X Expression System enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentivirus transduction. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious lentivirus particles. Lenti-X Packaging Single Shots, which contain lyophilized Lenti-X packaging plasmids and transfection reagent, are used for efficient introduction of the packaging mix and the pLVX-Puro Vector into 293T cells with very high efficiency. The concerted effects of these components allow 293T cells to produce high-titer replication incompetent lentiviral supernatants for a safe transduction of virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

631253 Lenti-X™ Expression System (EF1alpha Version) Each USD $1096.00

The Lenti-X Expression System (EF1α Version) enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentiviral transduction. The System contains an HIV-1-based, lentiviral expression vector, pLVX-EF1α-IRES-Puro, designed to constitutively express a protein of interest and puromycin resistance (Puror). Constitutive expression of these proteins is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows protein expression in mammalian cells in which the CMV promoter is silenced. The System also includes Lenti-X Packaging Single Shots (VSV-G) and Lenti-X GoStix.

631251 Lenti-X™ HTX Ecotropic Packaging System 20 Rxns USD $930.00

The Lenti-X HTX Ecotropic Packaging System allows you to produce very high virus titers from lentiviral vectors for the purposes of transferring exogenous genes into cells and tissues. The Lenti-X HTX Ecotropic Packaging Mix 2 is a proprietary combination of plasmids that expresses the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious, ecotropically-pseudotyped, lentiviral particles. The MLV ecotropic envelope glycoprotein enables mouse and rat cells to be transduced with high efficiency. The biodegradable nanoparticle-based Xfect transfection reagent transfers the packaging mix DNA and your lentiviral vector into Lenti-X 293T cells (Cat. No. 632180) with very high efficiency. It is the specific combination of these rigorously optimized components that allows your 293T cell cultures to produce extremely high titers of safe, replication-incompetent lentivirus from any of our Lenti-X System vectors, or from any other lentiviral vector. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus.

631235 Lenti-X™ qRT-PCR Titration Kit 200 Rxns USD $476.00

This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

3654 pDON-AI-2 DNA 20 ug USD $405.00

pDON-AI-2 DNA enables production of a retrovirus vector with high transfer efficiency. By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing virus-packaging signal (Ψ) and target gene. This vector possesses LTR and Ψ (viral packaging signal), but not the structural gene necessary for particle formation and replication, which are gag, pol, and env, and contains HCMV IE promoter within the U3 region of 5'LTR. The production of 2–8-fold higher-titer virus can be achieved by transfecting a version of pDON-AI-2 DNA containing a target gene compared using conventional retrovirus vector.

3653 pDON-AI-2 Neo DNA 20 ug USD $405.00

pDON-AI-2 DNA enables production of a retrovirus vector with high transfer efficiency. By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing virus-packaging signal (Ψ) and target gene. This vector possesses LTR and Ψ (viral packaging signal), but not the structural gene necessary for particle formation and replication, which are gag, pol, and env, and contains HCMV IE promoter within the U3 region of 5'LTR. pDON-AI-2 Neo DNA contains a neomycin resistant gene as a selective marker. The production of 2–8-fold higher-titer virus can be achieved by transfecting a version of pDON-AI-2 DNA containing a target gene compared using conventional retrovirus vector.

631453 Retro-X™ qRT-PCR Titration Kit 200 Rxns USD $476.00

This Retro-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of MMLV-based retroviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

631508 Retro-X™ System Each USD $789.00

The Retro-X System is a complete retroviral gene expression system for introducing stable heritable genetic material into mammalian cell lines. This system can be used to infect dividing cells from a broad range of mammalian cell lines.

631530 Retro-X™ Universal Packaging System Each USD $845.00

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.