PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time)

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Cat. # Product Size Price License Quantity Details
9160 EASY Dilution (for Real Time PCR) 8 x 1 mL USD $151.00

This product is used to dilute template DNA or RNA when preparing serial dilutions of the standard for establishing the standard curve in real time PCR.

RR390A Premix Ex Taq™ (Probe qPCR) 200 Rxns USD $270.00

Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses.

RR390B Premix Ex Taq™ (Probe qPCR) 400 Rxns USD $536.00

Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses. Cat. # RR390B contains 2 of Cat. # RR390A. Please refer to Cat. # RR390A for complete product documentation and resources.

RR036A PrimeScript™ RT Master Mix (Perfect Real Time) 200 Rxns USD $492.00

This product is a reverse transcription reagent kit designed to perform reverse transcription optimized for two step real-time RT-PCR (RT-qPCR). It contains a 5X pre-mixed reagent containing all of the components needed for quantitative RT-PCR reverse transcription (PrimeScript RTase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP Mixture, and reaction buffer), and a reaction can be started simply by adding template RNA and water. Because it uses PrimeScript RTase, which features excellent extension, this product makes it possible to synthesize template cDNA for real-time PCR efficiently in a short time. The cDNA obtained with this product can be used in both intercalating green dye qPCR assays and probe qPCR assays.

RR036B PrimeScript™ RT Master Mix (Perfect Real Time) 800 Rxns USD $1577.00

This product is a reverse transcription reagent kit designed to perform reverse transcription optimized for two step real-time RT-PCR (RT-qPCR). It contains a 5X pre-mixed reagent containing all of the components needed for quantitative RT-PCR reverse transcription (PrimeScript RTase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP Mixture, and reaction buffer), and a reaction can be started simply by adding template RNA and water. Because it uses PrimeScript RTase, which features excellent extension, this product makes it possible to synthesize template cDNA for real-time PCR efficiently in a short time. The cDNA obtained with this product can be used in both intercalating green dye qPCR assays and probe qPCR assays. Cat. # RR036B contains 4 of Cat. # RR036A. Please refer to Cat. # RR036A for complete product documentation and resources.

RR037A PrimeScript™ RT Reagent Kit (Perfect Real Time) 200 Rxns USD $328.00

PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.

RR037B PrimeScript™ RT Reagent Kit (Perfect Real Time) 800 Rxns USD $1042.00

PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B), for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe. Cat. # RR037B contains 4 of Cat. # RR037A. Please refer to Cat. # RR037A for complete product documentation and resources.

RR420A TB Green® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns USD $337.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

RR420B TB Green® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns USD $635.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat. # RR420B contains 2 of Cat. # RR420A. Please refer to Cat. # RR420A for complete product documentation and resources.

RR820A TB Green® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns USD $337.00

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNase H, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.

RR820B TB Green® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns USD $635.00

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNaseH, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that by TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.