The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit uses BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases, BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates. Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers, or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis.

The RNA PCR Kit (AMV) Version 3.0 is designed for performing single tube reverse transcription of RNA to cDNA using AMV (Avian Myeloblastosis Virus) Reverse Transcriptase, with subsequent cDNA amplification using Takara Ex Taq HS. Taking advantage of AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures (42–60°C) than MMLV RTase, this kit allows transcription at higher temperatures, which eliminates potential RNA secondary structure that can give rise to polymerase pauses. The supplied Oligo dT-Adaptor Primer is designed for highly efficient cDNA synthesis from the poly(A)⁺ RNA 3'-terminus, which allows amplification of unknown 3'-termini using 3'-RACE. The components for the 3'-RACE method are supplied in the kit.

The RNA LA PCR Kit is designed to generate longer and more accurate RT-PCR products. The kit uses AMV RT XL as the reverse transcriptase, which allows for efficient synthesis of first-strand cDNA up to 12 kb. AMV RT XL also provides better thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. In addition, the kit uses LA Taq, which can synthesize products up to 40 kb in size with 6.5X better fidelity than Taq Polymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. The components for 3'-RACE are also supplied.

Takara Bio's One Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL.