Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC