Guide-itâ„¢ CRISPR/Cas9 Gesicle Production System

Cas9 protein delivery in culture cells
The use of gesicles decreases off-target effects
Knockout efficiency of fluorescent reporter by Cas9-sgRNA protein complexes delivered to various cell types using gesicles
Gesicle production overview for delivery of Cas9-sgRNA
The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells
Editing efficiency is increased by an improved sgRNA scaffold design in gesicles
Using an improved sgRNA scaffold design in gesicles enables a dose-dependent increase in knockout effect
Optimal sgRNA design strategies when using gesicles
Efficient knockout of an endogenous protein (CD81) using gesicles containing Cas9-sgRNA complexes
Gesicles utilize an optimized sgRNA scaffold
Successful knockout of CD81 in hiPS cells