AAVpro® CRISPR/Cas9 Helper Free System (AAV2)

The AAVpro CRISPR/Cas9 system results in more mutations than transfection, especially in hard-to-transfect cell lines
Production of full-length Cas9 and sgRNA
AAV vector recombination to produce full-length Cas9 and sgRNA
Consistent recovery of equally high genomic titers of AAV2-Up and AAV2-Down viral particles
Vector map of pAAV-Guide-it-Up
Vector map of pAAV-Guide-it-Down
The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells