A complete filtration-based purification system for concentrating and purifying infectious adenovirus. The Adeno-X Maxi Purification Kit is an alternative to CsCl density gradient ultracentrifugation. Each kit provides the buffers and filters needed to purify adenovirus from cell lysate.

The Adeno-X Rapid Titer Kit takes advantage of production of viral hexon proteins for the titration of adenoviral stocks in 48 hours. A viral stock is tested by making serial dilutions, infecting HEK 293 cells, and 48 hours later, fixing and staining those cells with the Mouse Anti-Hexon Antibody. The signal is detected with a Rat Anti-Mouse Antibody conjugated to horseradish peroxidase (HRP) and developed with metal-enhanced 3,3'- Diaminobenzidine tetrahydrochloride (DAB).

Adeno-X Adenoviral System 3 (CMV) provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-CMV (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-CMV in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool.

Adeno-X GoStix are designed to instantly confirm the presence of adenovirus in cell culture supernatants or lysates. The test detects adenoviral hexon proteins and can be used to determine if infectious virus was produced post-transfection, or for selecting the best time to harvest your virus. The included Hexon Control provides confirmation of GoStix test function.

The Adeno-X 293 Cell Line is a low-passage transformed human embryonic kidney cell line for the production of adenovirus stocks. Adenovirus stocks can be produced by either transfecting this cell line with a linearized adenoviral vector (such as the linear vectors supplied with any Adeno-X System 3) or infecting with a viable adenovirus seed stock. The slower growth rate exhibited by this line reduces cell death due to overconfluence, and therefore facilitates more efficient rescue and amplification. These cells also possess strong adherence and are highly transfectable.