B. Subtilis Secretory Protein Expression System

Recommended Products

Cat. # Product Size Price License Quantity Details
3360 ◊pCold™ Vector Set 1 Set USD $1159.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

3362 ◊pCold™ II DNA 25 ug USD $441.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

1071A Mlu I 1,000 Units USD $61.00

DNA Restriction Enzymes from Takara such as MluI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases

3361 ◊pCold™ I DNA 25 ug USD $435.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli.  These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold IV DNA contains a translation enhancing element (TEE), his tag sequence, and a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

3363 ◊pCold™ III DNA 25 ug USD $441.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold III DNA also contains a his tag sequence and a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

3364 ◊pCold™ IV DNA 25 ug USD $441.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors (including pCold I) contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). pCold I also contains a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

636763 Stellar™ Competent Cells 10 x 100 uL USD $207.00

Stellar Competent Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to subcloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector is provided as well as SOC Medium. Stellar Competent Cells are recommended for use with Clontech's In-Fusion PCR Cloning Kits.