A high-capacity immobilized metal affinity chromatography resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

A high-capacity immobilized metal affinity chromatography resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

A high-capacity immobilized metal affinity chromatography resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions.

The Chaperone Plasmid Set consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a "chaperone team" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cm^r gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB or Pzt-1 (tet) promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary regulator (araC or tet^r) for each promoter.

Use this set to perform a two step method to construct target/chaperone co-expression systems: In the first step prepare an E. coli host transformed with only the chaperone plasmid. The second step is to prepare competent cells from this new strain and transform this strain with a plasmid expressing the target protein.

NOTE: For commercial use of this product, please refer to the protein expression vectors for commercial use page for ordering information. 

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions. xTractor Buffer is an optimized buffer system for extraction of proteins from fresh or frozen bacterial cells, mammalian cells, yeast cells, or baculovirus-infected cells. This buffer is useful in extracting proteins over a broad range of molecular weights, including high molecular weight proteins that cannot be extracted unless the membrane is fully disrupted. xTractor Buffer has been optimized for extraction of his-tagged proteins, so it is compatible with all IMAC resins. This buffer is suitable for any scale protein extraction & any tag (use with his-, GST-, HA- or other tagged proteins).

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions. The His60 Ni Buffer Set includes extraction, equilibration, and elution buffers optimized for the purification of his-tagged proteins on His60 Ni Superflow Resin and His60 Ni Gravity Columns.