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Home › Services & Support › Stem cell services › Research-grade stem cell services › Reprogramming

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Cellartis human pluripotent stem cell services

Reprogramming

Even if your lab does reprogramming, creating hiPS cell lines can be an inefficient process with heavy costs of time and resources. Additionally, creating an iPS cell line with your specific requirements is difficult due to the challenges inherent to the sourcing process. To make your iPS cell research easier on your team, we offer a reprogramming package to create iPS cells from your (or sourced) human PBMCs. If you already have your own fibroblasts, we can also reprogram them for you.

For more information about our services, please submit the service inquiry form below.

Overview Deliverables/lead time QC testing Project initiation

Overview  

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your PBMC or fibroblast samples. The cells will be reprogrammed using footprint-free Sendai virus technology to ensure no viral integration.

The reprogramming service can start with sending us your cell samples, or alternatively, you may elect to use Cellartis sourcing services to help you find exactly the right PBMCs to create a custom iPS cell line. Once the reprogramming service begins, the starting material will be prepared for reprogramming; one iPS cell clone will be established in the Cellartis DEF-CS 500 Culture System, then expanded into a stable iPS cell line, banked, and characterized.


Thawing of your (or sourced) cells, propagation as applicable
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Reprogramming using Sendai virus
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Picking of colonies
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Selection of clones and generation of seed banks
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Expansion of high-quality clone to Master Cell Bank (viral clearance confirmed)
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QC and characterization of Master Cell Bank (see QC testing section, below)
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Delivery of hiPS cell line to customer

Deliverables/lead time  

PBMC reprogramming

ServiceCat. #DeliverablesLead time
Cellartis Sendai Virus Reprogramming of PBMCs Service Y60102
  • Project report, including QC testing and characterization data
  • Master Cell Bank of one hiPS clone per donor (5 vials per clone with 2–3 million cells per vial)
  • License for internal research use
Approx. 20 weeks

Fibroblast reprogramming

ServiceCat. #DeliverablesLead time
Cellartis Sendai Virus Reprogramming of Fibroblasts Service Y60101
  • Project report, including QC testing and characterization data
  • Master Cell Bank of one hiPS clone per donor (5 vials per clone with 2–3 million cells per vial)
  • License for internal research use

Approx. 22 weeks

QC testing  

Included with the reprogramming service:

  • Visual inspection and confirmation of the morphologies of the cells in the starting material and in the Master Cell Bank
  • Immunocytochemistry of the cells in the Master Cell Bank to verify the presence of the pluripotency markers Oct-4, SSEA-4, TRA-1-60, and TRA-1-81, and the absence of SSEA-1
  • Sterility testing of the Master Cell Bank (bacteria, including Mycoplasma)
  • Confirmation that no Sendai virus vector remains in the cells

Additional testing and characterization services for reprogrammed cells:

  • Cellartis Descriptive Karyotyping Service (Cat. #Y60304)
    • Karyotype analysis of 20 G-banded metaphase spreads from reprogrammed cells will be performed.
  • Cellartis Spontaneous In Vitro Differentiation Service (Cat. #Y60305)
    • A 3-week spontaneous in vitro differentiation protocol will be used on a subset of reprogrammed cells. Cells will be fixed and immunolabelled for ASMA (mesoderm), FoxA2 (endoderm), and βIII-tubulin (ectoderm) to confirm trilineage potential.
  • Cellartis STR Analysis Service (Cat. #Y60306)
    • Genomic DNA will be isolated from reprogrammed iPS cells and the corresponding starting material. Short tandem repeat (STR) analysis will be performed on 16 loci to verify there are no differences between the samples.

Project initiation  

You may wish to submit your own fibroblast or PBMC samples for Cellartis reprogramming services or use Cellartis sourcing services to find custom starting materials. If you would like us to start with your cells, we will need:

  • Fibroblasts: 5 vials (1 million cells per vial) of customer-provided fibroblasts, with relevant information regarding culturing and freezing protocols
  • PBMCs: 5 vials (10 million cells per vial) of customer-provided PBMCs, with relevant information regarding culturing and freezing protocols

For safety and QC purposes, we can only work with human samples that are free of human pathogens and that have passed a sterility test. We require documentation proving that all samples shipped to us are sterile and pathogen-free. In the absence of documentation, testing for pathogens (Cat. #Y60301) and sterility (Cat. #Y60302) will be performed via a third-party service prior to the initiation of Cellartis services. Please note that safety and sterility testing will incur additional lead time and costs.

  1. Submit the service inquiry form below.
  2. We contact you to go over your project requirements and provide a quote.
  3. Place your order and ship us the necessary cells and documentation to initiate the service. (Does not apply if using our sourcing service.)
  4. We perform the agreed-upon service according to the designated lead times.
  5. We provide you with the agreed-upon deliverables.

    Note: Takara Bio Europe, AB (TBEAB) will perform the reprogramming service only one time per purchase. Each purchase of the reprogramming service includes one transduction attempt with Sendai virus per donor sample. If a successful reprogramming cannot be achieved after the transduction of the donor sample with Sendai virus, the reprogramming service will be terminated and neither TBUSA nor TBEAB will have any further obligation, including additional attempts of the reprogramming service. If additional work is required, TBUSA will discuss such work, pricing, and lead time with the customer.

Reprogramming services

Cat. # Product Size License Quantity Details
Y60301 Cellartis® Human Pathogen and Mycoplasma Testing Service Each USD $1485.00

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60304 Cellartis® Descriptive Karyotyping Service Each USD $3076.00

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60101 Cellartis® Sendai Virus Reprogramming of Fibroblasts Service Each USD $16457.00

License Statement

ID Number  
L62 This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 10,385,407 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This custom service is provided by Takara Bio Inc. based on a patent license from iPS Academia Japan, Inc. The use of iPSCs produced by this service (hereinafter referred to as the "custom iPSCs") is strictly limited to customer’s own internal research. Customer cannot have any right to use the custom iPSCs in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. There is no right to resell or transfer the custom iPSCs to a third party regardless of whether or not compensation is received.

The use of custom iPSCs is not allowed for the purposes of the intended use as described below: 1) Creation of a human clone through transplantation of the custom iPSCs-derived embryos into human or animal individuals, 2) Custom iPSCs transplantation into human embryos, 3) Custom iPSCs transplantation into human fetuses, and 4) Production of human embryos using the custom iPSCs-derived germ cells.

Customers wishing to use the custom iPSCs for purposes other than internal research use should contact us. No express or implied license is granted to the customer. Any use of the custom iPSCs for Commercial Purposes requires a patent license from iPS Academia Japan, Inc. "Commercial Purposes" means at least one of following activities including the use of custom iPSCs: 1) Use for manufacture of related products for sale including but not limited to culture medium or equipment, 2) Use in order to provide a service to a third party (including use of the differentiated cells from the custom iPSCs), 3) Use for screening compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use (including use of the differentiated cells from the custom iPSCs)*, and 4) Transfer to a third party (including transfer of the differentiated cells from the custom iPSCs). *A patent license to use the custom iPSCs and the use of the differentiated cells from the custom iPSCs for screening compounds may also be granted from Takara Bio Inc.
L71 This service is performed by Takara Bio Inc. and/or its affiliate(s) based on a commercial license to certain intellectual property rights held by ID Pharma Co.,Ltd. The deliverables and their use are covered by one or more claims of patents owned by ID Pharma, including PCT/JP00/03195, PCT/JP2009/062911, PCT/JP2011/069588 and PCT/JP2014/75248 and their foreign counterparts. The purchase of this service conveys to the buyer the non-transferable right to use the deliverables for their intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use the deliverables or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer the deliverables to a third party regardless of whether or not compensation is received. Purchase does not include any right to provide Contract Manufacturing Service with the use of the deliverables to a third party, unless a license is negotiated with ID Pharma Co., Ltd. Purchasers wishing to use the deliverables for purposes other than internal research use should contact us.

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60102 Cellartis® Sendai Virus Reprogramming of PBMCs Service Each Inquire for Quotation

License Statement

ID Number  
L62 This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 10,385,407 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This custom service is provided by Takara Bio Inc. based on a patent license from iPS Academia Japan, Inc. The use of iPSCs produced by this service (hereinafter referred to as the "custom iPSCs") is strictly limited to customer’s own internal research. Customer cannot have any right to use the custom iPSCs in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. There is no right to resell or transfer the custom iPSCs to a third party regardless of whether or not compensation is received.

The use of custom iPSCs is not allowed for the purposes of the intended use as described below: 1) Creation of a human clone through transplantation of the custom iPSCs-derived embryos into human or animal individuals, 2) Custom iPSCs transplantation into human embryos, 3) Custom iPSCs transplantation into human fetuses, and 4) Production of human embryos using the custom iPSCs-derived germ cells.

Customers wishing to use the custom iPSCs for purposes other than internal research use should contact us. No express or implied license is granted to the customer. Any use of the custom iPSCs for Commercial Purposes requires a patent license from iPS Academia Japan, Inc. "Commercial Purposes" means at least one of following activities including the use of custom iPSCs: 1) Use for manufacture of related products for sale including but not limited to culture medium or equipment, 2) Use in order to provide a service to a third party (including use of the differentiated cells from the custom iPSCs), 3) Use for screening compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use (including use of the differentiated cells from the custom iPSCs)*, and 4) Transfer to a third party (including transfer of the differentiated cells from the custom iPSCs). *A patent license to use the custom iPSCs and the use of the differentiated cells from the custom iPSCs for screening compounds may also be granted from Takara Bio Inc.
L71 This service is performed by Takara Bio Inc. and/or its affiliate(s) based on a commercial license to certain intellectual property rights held by ID Pharma Co.,Ltd. The deliverables and their use are covered by one or more claims of patents owned by ID Pharma, including PCT/JP00/03195, PCT/JP2009/062911, PCT/JP2011/069588 and PCT/JP2014/75248 and their foreign counterparts. The purchase of this service conveys to the buyer the non-transferable right to use the deliverables for their intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use the deliverables or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer the deliverables to a third party regardless of whether or not compensation is received. Purchase does not include any right to provide Contract Manufacturing Service with the use of the deliverables to a third party, unless a license is negotiated with ID Pharma Co., Ltd. Purchasers wishing to use the deliverables for purposes other than internal research use should contact us.
*

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60305 Cellartis® Spontaneous In Vitro Differentiation Service Each USD $3464.00

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60302 Cellartis® Sterility Testing Service Each USD $711.00

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60306 Cellartis® STR Analysis Service Each USD $952.00

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
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  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
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