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  • ‹ Back to Stem cell services
  • Clinical-grade hES cell line derivation
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  • Directed differentiation to hepatocytes
  • Directed differentiation to beta cells
  • Directed differentiation to definitive endoderm cells
  • Human pathogen and sterility testing

Tagging an endogenous gene with AcGFP1 in hiPS cells Case study: Tagging an endogenous gene with a fluorescent tag
Tagging an endogenous gene with an epitope tag Case study: Tagging an endogenous gene with an epitope tag
Knocking out an endogenous gene (CD81) in hiPS cells Case study: Knocking out an endogenous gene
Introducing a disease-related SNP in hiPS cells Case study: Introducing a disease-related SNP
Inserting an expression cassette into a specific locus Case study: Knocking in an expression cassette
Analysis of a culture flask Human pathogen and sterility testing service
Home › Services & Support › Instrument & reagent services › Stem cell services › Gene editing

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Tagging an endogenous gene with AcGFP1 in hiPS cells Case study: Tagging an endogenous gene with a fluorescent tag
Tagging an endogenous gene with an epitope tag Case study: Tagging an endogenous gene with an epitope tag
Knocking out an endogenous gene (CD81) in hiPS cells Case study: Knocking out an endogenous gene
Introducing a disease-related SNP in hiPS cells Case study: Introducing a disease-related SNP
Inserting an expression cassette into a specific locus Case study: Knocking in an expression cassette
Analysis of a culture flask Human pathogen and sterility testing service
Cellartis Human Pluripotent Stem Cell Services

Gene editing

The combination of precise, footprint-free gene editing using CRISPR/Cas9 and human pluripotent stem cells allows for the generation of disease models that are essential for advancing our understanding of disease mechanisms and the development of novel therapeutics. However, creating these models can be extremely challenging as successful gene editing and concurrent establishment of clonal populations can be both inefficient and time-consuming. To help you create your unique disease models, we offer gene editing services to knock out a target gene or knock in a disease-specific mutation. With the help of our services, you can focus on your research and leave the challenges to us.

For more information about our services, please submit the service inquiry form below.

Overview Project types Deliverables/lead time QC testing Project initiation

Overview  

With Cellartis gene editing services, we will deliver high-quality, edited human iPS cells from your disease- or patient-specific donor lines. The cells will be edited according to your specifications using CRISPR/Cas9 technology.

The gene editing services start with you sending us your own human iPS cells. As an alternative, you may request the Cellartis sourcing and reprogramming services. Our gene editing protocol starts with thawing and adapting your pluripotent cells to the Cellartis DEF-CS 500 Culture System, and concurrent design and production of sgRNAs and ssDNA for knockins. The cells are then edited via electroporation-based delivery of ribonucleoprotein (RNP) complexes, screened, and single-cell cloned. Finally, clones of interest are identified, expanded, and characterized. Depending on your downstream applications, you may request the Cellartis directed differentiation services for your edited cells.

Thawing of your human iPS cells and adaptation to the DEF-CS system
sgRNA and ssDNA design and production

Gene editing via RNP electroporation

Screening of edited populations

Single-cell cloning

Screening of clones and identification of clones of interest

Expansion of clones and creation of Master Cell Banks

Characterization of Master Cell Banks (QC testing included)

Project types  

Service What kind Alleles Affected Zygosity Notes Case study
Knockout Out-of-frame knockout (first exon) monoallelic heterozygous Knocking out an endogenous gene (CD81) in hiPS cells
biallelic heterozygous
Protein truncation monoallelic heterozygous
biallelic heterozygous
Knockin SNP monoallelic heterozygous Second allele most likely knocked out and not wild type Introducing a tyrosinemia-related SNP in hiPS cells
biallelic homozygous
Gene-tagging (longer sequences up to 4 kb; fluorescent proteins, FLAG, etc.) monoallelic heterozygous Second allele most likely knocked out and not wild type

Tagging an endogenous gene with a myc tag in hiPS cells

Tagging an endogenous gene with AcGFP1 in hiPS cells

biallelic homozygous
Complete expression cassette monoallelic heterozygous Second allele most likely knocked out and not wild type Inserting an expression cassette into the AAVS1 locus in hiPS cells
biallelic homozygous

Deliverables/lead time  

Service Cat. # Deliverables Lead time
Cellartis Human iPS Gene Editing Service (Knockout)* Y60120
  • Service report, including characterization data
  • 3 edited clones
  • 5 vials per clone (3 million cells per vial)
Approx. 26 weeks
Cellartis Human iPS Gene Editing Service (Knockin)* 
Y60121
  • Service report, including characterization data
  • 3 edited clones
  • 5 vials per clone (3 million cells per vial)
Approx. 28 weeks

*If you are interested in gene editing services for human embryonic stem cells, please contact us by submitting the form below.

QC testing  

Included with gene editing services:

  • Visual inspection and confirmation of cell morphology and proliferation
  • Immunocytochemistry to verify the presence of the pluripotency markers Oct-4, SSEA-4, TRA-1-60, and TRA-1-81
  • Sequence analysis
  • Sterility testing of the final cell product (bacteria, including Mycoplasma)

Additional testing and characterization services for edited cells:

  • Cellartis Descriptive Karyotyping Service (Cat. # Y60304)
    • Karyotype analysis of 20 G-banded metaphase spreads from edited cells will be performed.
  • Cellartis Spontaneous In Vitro Differentiation Service (Cat. # Y60305)
    • A 3-week spontaneous in vitro differentiation protocol will be used on a subset of edited cells. Cells will be fixed and immunolabelled for ASMA (mesoderm), FoxA2 (endoderm), and βIII-tubulin (ectoderm) to confirm trilineage potential.
  • Cellartis STR Analysis Service (Cat. # Y60306)
    • Genomic DNA will be isolated from edited cells and the corresponding starting material. Short tandem repeat (STR) analysis will be performed on 16 loci to verify there are no differences between the samples.

Project initiation  

You may wish to submit your own human iPS cells for Cellartis gene editing services, or use Cellartis sourcing and reprogramming services to generate the starting material. If you would like to start with your cells, we will need 5 vials (>1 million viable cells per vial) of hiPS cells, with relevant information, including name of the donor cell line, culture conditions, and cell density upon freezing. Your cells will be first adapted to the DEF-CS system, and then the editing protocol can begin.

For safety and QC purposes, we can only work with human samples that are free of human pathogens and that have passed a sterility test. We require documentation proving that all samples shipped to us are sterile and pathogen-free. In the absence of documentation, testing for pathogens (Cat. # Y60301) and sterility (Cat. # Y60302) will be performed via a third-party service prior to the initiation of Cellartis services. Please note that safety and sterility testing will incur additional lead time and costs.

  1. Submit the service inquiry form below.
  2. We contact you to go over your project requirements and provide a quote.
  3. Place your order and ship us the necessary cells and documentation to initiate the service. (Does not apply if using our sourcing service.)
  4. We perform the agreed-upon service according to the designated lead times.
  5. We provide you with the agreed-upon deliverables.


Contact us about stem cell services

Please submit your information, and we will contact you to discuss your needs. 

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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