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Showing 41-50 of 1190 results

CellAmp™ RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis

Cat. # Product Size License Quantity Details
3733 CellAmp™ RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis 200 Rxns USD $273.00

The CellAmp Direct RNA Prep Kit for qRT-PCR and Protein Analysis allows simple, direct extraction directly from cultured cells to prepare templates used for one-step and two-step qRT-PCR. The kit can be used for cells cultured in 96-well plates and is compatible with high-throughput studies. Preparation of templates for real-time PCR from cultured cells takes approximately 10 minutes and complete gene expression analysis (via One Step or Two Step qRT-PCR) is performed in approximately 2 hours. When used in combination with a reverse transcription kit, cDNA templates for real-time PCR can be synthesized in about 30 minutes, providing efficient high-throughput analysis. Templates can be prepared directly from small quantities of cells for analysis by high sensitivity qRT-PCR. Genomic DNA can be easily and efficiently removed using this kit. Excellent for detection of rare transcripts.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

HAT™ Protein Expression & Purification System

Cat. # Product Size License Quantity Details
631205 HAT™ Protein Expression & Purification System Each USD $695.00

This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT
sequence and EK site are in frame with the lacZ initiation codon. The vector construct is provided in all three reading frames.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Protein ladder sets: CLEARLY Protein Ladder molecular weight markers

The CLEARLY Protein Ladder sets are ready-to-run protein molecular weight standards for SDS-PAGE. Use these protein marker sets for protein electrophoresis and Western blotting.

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Tissue-specific protein extracts

Comprehensive collection of high-quality human total protein extracts to quickly confirm tissue-specific abundance of your protein.

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Soluble protein expression: single-protein production system

The SPP (single-protein production) system utilizes a modified version of Takara's cold-shock expression vectors along with expression of the endoribonuclease MazF to achieve remarkable protein purity.

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B. subtilis Secretory Protein Expression System

Use the B. Subtilis Protein Expression System for high yields of soluble, active recombinant protein secreted directly into the culture media.

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Protein detection using his-tag antibodies and an HA tag antibody

Three different his-tag antibodies and an HA-tag antibody for highly sensitive and specific protein detection in Western, ELISA, and IHC assays.

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Straightforward RNA isolation: CellAmp direct RNA prep kit for qPCR or protein analysis

Prepare cell lysate from cultured animal cells in just 10 minutes, and use it directly for either analysis of gene expression by real-time RT-PCR or protein expression by western blot.

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Rapid Protein G-based antibody purification (Phase-out notice: These products will not be available after current inventory is exhausted)

High-capacity membrane technology with immobilized Protein G in spin column and filtration device formats for antibody purification.

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Recombinant protein expression: Brevibacillus expression systems

The Brevibacillus expression systems generate secreted target proteins efficiently and is ideal for eukaryotic recombinant protein expression, resulting in high yields of active protein.

Showing 41-50 of 1190 results