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Showing 1-10 of 18 results

pHcRed1 Vector

Cat. # Product Size License Quantity Details
632410 pHcRed1 Vector 20 ug USD $589.00

pHcRed1 is a bacterial expression vector which encodes the far-red fluorescent protein, HcRed1. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa. The HcRed1 coding sequence has been human codon-optimized for higher expression in mammalian cells. The sequence upstream of HcRed1 has been converted to a Kozak consensus sequence to further increase the translation efficiency in eukaryotic cells.

The HcRed1 coding sequence is flanked by distinct multiple cloning sites (MCS) so that the gene can be easily excised from pHcRed1. Alternatively, the HcRed1 coding sequence can be amplified by PCR. In E. coli, HcRed1 is expressed from the lac promoter as a fusion with several additional amino acids, including the first five amino acids of the LacZ protein. Note, however, that if you excise the HcRed1 coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e., non-fusion) HcRed1 protein. pHcRed1 is a pUC 19 derivative (pPD16.43) which provides a high-copy-number origin of replication as well as an ampicillin resistance gene for propagation and selection in E. coli.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed2 Vector

Cat. # Product Size License Quantity Details
632404 pDsRed2 Vector 20 ug USD $589.00

pDsRed2 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. In pDsRed2, the DsRed2 coding sequence is flanked by two separate MCS regions at the 5' and 3' ends, making it possible to excise and shuttle the DsRed2 coding region into other expression vectors. pDsRed2 is intended to serve as a source of the DsRed2 cDNA; It can also be used in bacteria to produce DsRed2 protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pAcGFP1 Vector

Cat. # Product Size License Quantity Details
632468 pAcGFP1 Vector 20 ug USD $589.00

pAcGFP1 encodes a monomeric green fluorescent protein from Aequorea coerulescens. pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed-Express Vector

Cat. # Product Size License Quantity Details
632412 pDsRed-Express Vector 20 ug USD $589.00

pDsRed-Express is a prokaryotic expression vector that encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). pDsRed-Express is primarily intended to serve as a source of DsRed-Express cDNA. The flanking MCS regions make it possible to excise the DsRed-Express coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express protein.

DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the time from transfection to detection of red fluorescence, and decrease the level of residual green emission. When DsRed-Express is expressed in mammalian cell cultures, red-emitting cells can be detected by either fluorescence microscopy or flow cytometry 8–12 hours after transfection. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. The DsRed-Express coding sequence is human codon-optimized for high expression in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed2-Bid Vector

Cat. # Product Size License Quantity Details
632419 pDsRed2-Bid Vector 20 ug USD $589.00

pDsRed2-Bid encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and Bid, a member of the Bcl-2 family. In healthy, non-apoptotic cells, Bid resides in the cytosol as soluble protein. Upon induction of apoptosis, Bid translocates to mitochondria. In cells expressing the Bid-DsRed2 fusion, the translocation can be detected by fluorescence microscopy.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pmOrange2 Vector

Cat. # Product Size License Quantity Details
632548 pmOrange2 Vector 20 ug USD $589.00

pmOrange2 is a Living Colors fluorescent protein vector which encodes mOrange2, a variant of mOrange which has been modified for improved stability. mOrange2 is derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mOrange2 coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmOrange2 is primarily intended to serve as a source of mOrange2 cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pE2-Crimson Vector

Cat. # Product Size License Quantity Details
632553 pE2-Crimson Vector 20 ug USD $589.00

pE2-Crimson is a prokaryotic expression vector which encodes E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. pE2-Crimson is primarily intended to serve as a source of E2-Crimson cDNA. The flanking MCS regions make it possible to excise the E2-Crimson coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce E2-Crimson protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed-Express2 Vector

Cat. # Product Size License Quantity Details
632535 pDsRed-Express2 Vector 20 ug USD $571.00

pDsRed-Express2 is a prokaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. pDsRed-Express 2 is primarily intended to serve as a source of DsRed-Express 2 cDNA. The flanking MCS regions make it possible to excise the DsRed-Express2 coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express2 protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pmPlum Vector

Cat. # Product Size License Quantity Details
632527 pmPlum Vector 20 ug USD $529.00

pmPlum encodes mPlum, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mPlum coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmPlum is primarily intended to serve as a source of mPlum cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pAsRed2 Vector

Cat. # Product Size License Quantity Details
632451 pAsRed2 Vector 20 ug USD $589.00

pAsRed2 is a prokaryotic expression vector, which encodes a human codon-optimized variant of Anemonia sulcata red fluorescent protein, AsRed2, which has been engineered for brighter fluorescence and higher expression in mammalian cells. In this vector, the AsRed2 gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the AsRed2 gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of AsRed2 cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 18 results