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Showing 1-10 of 13 results

pDsRed2-1 Vector

Cat. # Product Size License Quantity Details
632405 pDsRed2-1 Vector 20 ug USD $589.00

pDsRed2-1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. pDsRed2-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless DsRed2 gene. Without the addition of a functional promoter, this vector will not express DsRed2.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pAcGFP1-1 Vector

Cat. # Product Size License Quantity Details
632497 pAcGFP1-1 Vector 20 ug USD $589.00

pAcGFP1-1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells. The vector contains a neomycin resistance gene for the selection of stable transfectants. pAcGFP1-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless AcGFP1 gene. Without the addition of a functional promoter, this vector will not express AcGFP1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pEF1alpha-IRES-ZsGreen1 Vector

Cat. # Product Size License Quantity Details
631976 pEF1alpha-IRES-ZsGreen1 Vector 10 ug USD $555.00

pEF1α-IRES-ZsGreen1 is a bicistronic mammalian expression vector that allows the simultaneous, constitutive expression of a protein of interest and the green fluorescent protein ZsGreen1. Constitutive expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pEF1alpha-IRES-DsRed-Express2 Vector

Cat. # Product Size License Quantity Details
631980 pEF1alpha-IRES-DsRed-Express2 Vector 10 ug USD $555.00

pEF1α-IRES-DsRed-Express2 is a bicistronic mammalian expression vector that allows the simultaneous, constitutive expression of a protein of interest and the red fluorescent protein DsRed-Express2. Expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed-Express-1 Vector

Cat. # Product Size License Quantity Details
632413 pDsRed-Express-1 Vector 20 ug USD $589.00

pDsRed-Express-1 is a promoterless mammalian expression vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into MCS. It encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the time from transfection to detection of red fluorescence, and decrease the level of residual green emission. When DsRed-Express is expressed in mammalian cell cultures, red-emitting cells can be detected by either fluorescence microscopy or flow cytometry 8–12 hours after transfection. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. The DsRed-Express coding sequence is human codon-optimized for high expression in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pHcRed1-1 Vector

Cat. # Product Size License Quantity Details
632411 pHcRed1-1 Vector 20 ug USD $589.00

pHcRed1-1 is a promoterless, mammalian expression vector which can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS) located upstream of the HcRed1 coding sequence. Promoters should be cloned into the pHcRed1-1 MCS upstream from the HcRed1 coding sequences. Without the addition of a functional promoter, this vector will not express HcRed1.

HcRed1 is a far-red fluorescent protein which was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa. The HcRed1 coding sequence has been human codon-optimized for higher expression in mammalian cells. The sequence upstream of HcRed1 has been converted to a Kozak consensus sequence to further increase the translation efficiency in eukaryotic cells. SV40 polyadenylation signals downstream of the HcRed1 gene direct proper processing of the 3' end of the HcRed1 mRNA. The vector also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette (Neor)-consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene-allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli.

pHcRed1-1 can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pmCherry-1 Vector

Cat. # Product Size License Quantity Details
632525 pmCherry-1 Vector 20 ug USD $529.00

pmCherry-1 encodes mCherry, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. pmCherry-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS). Promoters should be cloned into the pmCherry-1 MCS upstream of the mCherry coding sequence. Without the addition of a functional promoter, this vector will not express mCherry.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pZsGreen1-1 Vector

Cat. # Product Size License Quantity Details
632473 pZsGreen1-1 Vector 20 ug USD $589.00

pZsGreen1-1 encodes ZsGreen1, a variant of the Zoanthus sp. green fluorescent protein (ZsGreen1) that has been engineered for brighter fluorescence. The ZsGreen1 coding sequence contains a series of silent base pair changes for optimal expression in mammalian cells. pZsGreen1-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless ZsGreen1 gene. Without the addition of a functional promoter, this vector will not express ZsGreen1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pDsRed-Express2-1 Vector

Cat. # Product Size License Quantity Details
632536 pDsRed-Express2-1 Vector 20 ug USD $571.00

pDsRed-Express2-1 encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. pDsRed-Express2-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS) upstream of the promoterless DsRed-Express2 gene. Without the addition of a functional promoter, this vector will not express DsRed-Express2.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pHcRed1-DR Vector

Cat. # Product Size License Quantity Details
632422 pHcRed1-DR Vector 20 ug USD $589.00

pHcRed1-DR is a promoterless vector that encodes HcRed1-DR, a destabilized variant of Heteractis crispa far-red fluorescent protein, HcRed1. HcRed1-DR contains a PEST degradation domain at its C-terminus, so it is rapidly turned over in mammalian cell culture. Because if its short half-life, HcRed1-DR is well suited for monitoring the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site, located upstream of the human codon-optimized HCRed1-DR gene. Without the addition of a functional promoter, this vector will not express HcRed1-DR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 13 results