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pET Express & Purify Kit—His60 (In-Fusion® Ready)

Cat. # Product Size License Quantity Details
631428 pET Express & Purify Kit—His60 (In-Fusion® Ready) 20 Purifications USD $843.00

The pET Express & Purify Kit-His60 (In-Fusion Ready) allows you to use In-Fusion cloning technology to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system contains IPTG-inducible, pET-based bacterial expression vectors that have been prelinearized to allow In-Fusion cloning via the included In-Fusion HD Enzyme Premix. These vectors contain a T7/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins, which can be easily purified with the high-capacity His60 Ni Gravity Columns included in the kit. The kit also contains a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Resin & xTractor™ Buffer Bundle

Cat. # Product Size License Quantity Details
635676 His60 Ni Superflow Resin & xTractor™ Buffer Bundle 20 Purifications USD $289.00

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions. xTractor Buffer is an optimized buffer system for extraction of proteins from fresh or frozen bacterial cells, mammalian cells, yeast cells, or baculovirus-infected cells. This buffer is useful in extracting proteins over a broad range of molecular weights, including high molecular weight proteins that cannot be extracted unless the membrane is fully disrupted. xTractor Buffer has been optimized for extraction of his-tagged proteins, so it is compatible with all IMAC resins. This buffer is suitable for any scale protein extraction & any tag (use with his-, GST-, HA- or other tagged proteins).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Cartridge Purification Kit

Cat. # Product Size License Quantity Details
635674 His60 Ni Superflow Cartridge Purification Kit 5 x 1 mL Cartridges USD $539.00

Prepacked cartridges, each containing 1 ml of His60 Ni Superflow Resin, for the purification of recombinant his-tagged proteins. The cartridges are suitable for automated purification using medium-pressure systems such as ÄKTA FPLC or manual, syringe-based purification. Proteins can be purified under native or denaturing conditions. Each column can be reused up to 5 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Cartridges

Cat. # Product Size License Quantity Details
635675 His60 Ni Superflow Cartridges 5 x 1 mL Cartridges USD $259.00

This kit provides 5 prepacked cartridges, each containing 1 ml of His60 Ni Superflow Resin, and all of the buffers necessary to purify recombinant his-tagged proteins. The cartridges are suitable for automated purification using medium-pressure systems such as ÄKTA FPLC or manual, syringe-based purification. Proteins can be purified under native or denaturing conditions. Each column can be reused up to 5 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Cartridge Purification Kit

Cat. # Product Size License Quantity Details
635678 His60 Ni Superflow Cartridge Purification Kit 5 x 5 mL Cartridges USD $872.00

This kit provides 5 prepacked cartridges, each containing 5 ml of His60 Ni Superflow Resin, and all of the buffers necessary to purify recombinant his-tagged proteins. The cartridges are suitable for automated purification using medium-pressure systems such as ÄKTA FPLC or manual, syringe-based purification. Proteins can be purified under native or denaturing conditions. Each column can be reused up to 5 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Cartridges

Cat. # Product Size License Quantity Details
635679 His60 Ni Superflow Cartridges 5 x 5 mL Cartridges USD $661.00

Prepacked cartridges, each containing 5 ml of His60 Ni Superflow Resin, for the purification of recombinant his-tagged proteins. These cartridges are suitable for automated purification using medium-pressure systems such as ÄKTA FPLC or manual, syringe-based purification. Proteins can be purified under native or denaturing conditions. Each column can be reused up to 5 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Cartridge

Cat. # Product Size License Quantity Details
635680 His60 Ni Superflow Cartridge 1 x 5 mL Cartridge USD $155.00

Prepacked cartridges, each containing 5 ml of His60 Ni Superflow Resin, for the purification of recombinant his-tagged proteins. These cartridges are suitable for automated purification using medium-pressure systems such as ÄKTA FPLC or manual, syringe-based purification. Proteins can be purified under native or denaturing conditions. Each column can be reused up to 5 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Gravity Columns

Cat. # Product Size License Quantity Details
635657 His60 Ni Gravity Columns 5 x 1 mL Columns USD $132.00

Each prepacked His60 Ni Gravity Column contains 1 ml of His60 Ni Superflow Resin, a high capacity resin for the purification of recombinant his-tagged proteins. Suitable for gravity-flow purification under native or denaturing conditions, each column can be reused 4 times to purify different preparations of the same protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Gravity Column Purification Kit

Cat. # Product Size License Quantity Details
635658 His60 Ni Gravity Column Purification Kit 5 x 1 mL Columns USD $359.00

The His60 Ni Gravity Column Purification Kit provides prepacked, high-capacity His60 Ni Gravity Columns and all of the buffers necessary to purify recombinant his-tagged proteins. This kit is suitable for gravity flow purification under native or denaturing conditions. Each column contains 1 ml of His60 Ni Superflow Resin, and can be reused 4 times to purify different preparations of the same protein. The kit provides enough reagents for 20 purifications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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His60 Ni Superflow Resin & Buffer Set Bundle

Cat. # Product Size License Quantity Details
635677 His60 Ni Superflow Resin & Buffer Set Bundle 20 Purifications USD $448.00

His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions. The His60 Ni Buffer Set includes extraction, equilibration, and elution buffers optimized for the purification of his-tagged proteins on His60 Ni Superflow Resin and His60 Ni Gravity Columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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