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Showing 1-10 of 38 results

pTRE-Cycle2 Vector

Cat. # Product Size License Quantity Details
631116 pTRE-Cycle2 Vector 20 ug USD $561.00

pTRE-Cycle2 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the red fluorescent protein mCherry. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi. Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. mCherry is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pTRE-Cycle3 Vector

Cat. # Product Size License Quantity Details
631117 pTRE-Cycle3 Vector 20 ug USD $561.00

pTRE-Cycle3 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the fluorescent protein ZsGreen1. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi.Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. ZsGreen1 is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)

Cat. # Product Size License Quantity Details
631341 Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, mCherry) Each USD $1095.00

The Tet-On 3G Inducible Expression System (EF1alpha, mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)

Cat. # Product Size License Quantity Details
631342 Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1) Each USD $1095.00

The Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a green fluorescent protein (ZsGreen1) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-ZsGreen1 Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the ZsGreen1 marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Tet-On® 3G Inducible Expression System (EF1alpha, mCherry)

Cat. # Product Size License Quantity Details
631347 Tet-On® 3G Inducible Expression System (EF1alpha, mCherry) Each USD $1095.00

The Tet-On 3G Inducible Expression System (EF1alpha, mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker under the control of a Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-mCherry Vector Set, consisting of an IRES-based vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a control vector that inducibly expresses luciferase, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Tet-On® 3G Inducible Expression System (EF1alpha, ZsGreen1)

Cat. # Product Size License Quantity Details
631348 Tet-On® 3G Inducible Expression System (EF1alpha, ZsGreen1) Each USD $1095.00

The Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a green fluorescent protein (ZsGreen1) marker under the control of a Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-ZsGreen1 Vector Set, consisting of an IRES-based vector into which the gene of interest can be cloned and simultaneously expressed with the ZsGreen1 marker, a control vector that inducibly expresses luciferase, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Tet-On® 3G Bidirectional Inducible Expression System (with mCherry)

Cat. # Product Size License Quantity Details
631338 Tet-On® 3G Bidirectional Inducible Expression System (with mCherry) Each USD $1095.00

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Tet-On® 3G Bidirectional Inducible Expression System (with ZsGreen1)

Cat. # Product Size License Quantity Details
631339 Tet-On® 3G Bidirectional Inducible Expression System (with ZsGreen1) Each USD $1095.00

The Tet-On 3G Inducible Expression System (with ZsGreen1) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a green fluorescent protein (ZsGreen1) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-ZsGreen1 Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the ZsGreen1 marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Lenti-X™ Tet-On® 3G Inducible Expression System (with mCherry)

Cat. # Product Size License Quantity Details
631349 Lenti-X™ Tet-On® 3G Inducible Expression System (with mCherry) Each USD $1768.00

The Lenti-X Tet-On 3G Inducible Expression System (with mCherry) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system in both dividing and non-dividing cell types. pLVX-TRE3G-mCherry contains an internal ribosome entry site (IRES), allowing the simultaneous induced expression of a gene of interest and a red fluorescent protein marker from the same RNA transcript. The pLVX-Tet3G Vector expresses the Tet-On 3G transactivator protein from a CMV promoter. The kit also includes a complete fourth generation lentiviral packaging system, Lenti-X Packaging Single Shots (VSV-G).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Lenti-X™ Tet-On® 3G Inducible Expression System (with ZsGreen1)

Cat. # Product Size License Quantity Details
631350 Lenti-X™ Tet-On® 3G Inducible Expression System (with ZsGreen1) Each USD $1768.00

The Lenti-X Tet-On 3G Inducible Expression System (with ZsGreen1) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system in both dividing and non-dividing cell types. pLVX-TRE3G-ZsGreen1 contains an internal ribosome entry site (IRES), allowing the simultaneous induced expression of a gene of interest and a green fluorescent protein marker from the same RNA transcript. The pLVX-Tet3G Vector expresses the Tet-On 3G transactivator protein from a CMV promoter. The kit also includes a complete fourth generation lentiviral packaging system, Lenti-X Packaging Single Shots (VSV-G).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Showing 1-10 of 38 results