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Showing 1-10 of 23 results

pGADT7 AD Vector

Cat. # Product Size License Quantity Details
630442 pGADT7 AD Vector 20 ug USD $453.00

pGADT7 AD is a yeast expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. To facilitate in vitro transcription/translation, pGADT7 AD includes the T7 promoter between the GAL4 AD and the epitope tag.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pGBKT7 DNA-BD Vector

Cat. # Product Size License Quantity Details
630443 pGBKT7 DNA-BD Vector 20 ug USD $453.00

pGBKT7 is a yeast expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the medium ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pBridge Vector

Cat. # Product Size License Quantity Details
630404 pBridge Vector 20 ug USD $453.00

This vector allows expression of two proteins: a DNA-binding domain fusion, and an additional protein under control of an inducible methionine promoter. In combination with an activation domain fusion and reporter strain from a GAL4-based two-hybrid system, one can establish a three hybrid system for the investigation of ternary protein complexes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Matchmaker® Gold Yeast Two-Hybrid System

Cat. # Product Size License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y2HGold Yeast Strain

Cat. # Product Size License Quantity Details
630498 Y2HGold Yeast Strain 0.5 mL USD $591.00

The Saccharomyces cerevisiae strain Y2HGold is designed for use with Takara Bio's Matchmaker Gold Yeast Two-Hybrid System. Y2HGold includes the sensitive Aureobasidin A antibiotic resistance gene as one of four reporters—providing exceptionally stringent yeast two-hybrid (Y2H) screening. This strain contains distinct ADE2, HIS3, MEL1, and AUR1-C reporter constructs that are only expressed in the presence of GAL4-based protein interactions. Y2HGold is extremely effective in minimizing false positive protein interactions and background during a typical GAL4-based two-hybrid screen.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Matchmaker® Mammalian Assay Kit 2

Cat. # Product Size License Quantity Details
630305 Matchmaker® Mammalian Assay Kit 2 Each USD $790.00

Complete kit for confirming a known protein-protein interaction using two-hybrid technology in mammalian cell culture. Protein-protein interaction is assayed by measuring SEAP gene expression. No lysis is required.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631736 Great EscAPe™ SEAP Chemiluminescence Kit 2.0 50 Rxns USD $261.00

The Great EscAPe SEAP Chemiluminescence Kit 2.0 is a complete system for the detection of secreted alkaline phosphatase (SEAP) activity in the growth media from mammalian cells expressing this enzyme. The kit contains reagents necessary for the chemiluminescent detection of SEAP activity using a 96-well plate luminometer. The assay can also be performed in single tubes and measured in a tube luminometer. It allows you to monitor promoter activity over time, because samples of the cell media can be taken repeatedly and tested without lysing cells.

Mate & Plate™ Library - HeLa S3 (Normalized)

Cat. # Product Size License Quantity Details
630479 Mate & Plate™ Library - HeLa S3 (Normalized) 5 x 1 mL USD $1263.00

This yeast two-hybrid library was constructed from HeLa S3 cDNA that had been previously normalized to preferentially remove abundant cDNAs derived from high-copy-number mRNAs. The normalization process incorporates a Duplex-Specific Nuclease (DSN) treatment and SMART technology, and increases the representation of low-copy-number transcripts in the library. This reduces the number of clones that must be screened to identify positive interactions, and facilitates the identification and characterization of novel protein-protein interactions.

HeLa S3 cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATaGAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Mate & Plate™ Library - Mouse Embryonic Stem Cell (Normalized)

Cat. # Product Size License Quantity Details
630484 Mate & Plate™ Library - Mouse Embryonic Stem Cell (Normalized) 5 x 1 mL USD $1263.00

This yeast two-hybrid library was constructed from mRNA isolated from mouse embryonic stem cells (E14TG2A cell line) and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low-copy-number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMART technology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions.

The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Mate & Plate™ Library - Universal Drosophila (Normalized)

Cat. # Product Size License Quantity Details
630485 Mate & Plate™ Library - Universal Drosophila (Normalized) 5 x 1 mL USD $1263.00

This yeast two-hybrid library was constructed from mRNA isolated from Drosophila melanogaster and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low copy number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMART technology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions.The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Mate & Plate™ Library - Human Brain (Normalized)

Cat. # Product Size License Quantity Details
630486 Mate & Plate™ Library - Human Brain (Normalized) 5 x 1 mL USD $1263.00

This yeast two-hybrid library was constructed from mRNA isolated from human brain tissue and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low copy number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMART technology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions. The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Showing 1-10 of 23 results