The Human Vitronectin EIA Kit can detect two types of VN protein (65 and 75 kD) in blood using monoclonal antibodies. It can be also used for VN detection in urine and cell culture supernatant. In addition, this kit can also detect vitronectin in rabbit. Because the antibody in this kit does not cross-react with bovine vitronectin antigens, cell culture medium containing fetal bovine serum can be measured directly.

Back

MK103: Human Vitronectin EIA Kit

Takara's Heparan Degrading Enzyme Assay Kit provides a 96-well non-radioactive assay for the measurement of heparan degrading enzyme activity in cultured cells, tissues, or serum as well as screening of heparan sulfate degrading enzyme inhibitors. This kit is based upon the property of heparin-like molecules to bind bFGF (basic fibroblast growth factor). When heparan sulfate is degraded by heparan sulfate degrading enzyme, it loses its ability to bind to bFGF. Thus, the enzymic activity of a sample can be quantitated by comparing the amount of bFGF-bound undegraded heparan sulfate of the sample to total-bound heparan sulfate of a heparanase-free control. A unique feature of this kit is the use of CBD-FGF, a fusion protein composed of human fibronectin cell-binding domain and human fibroblast growth factor. CBD-FGF is immobilized on the surface of Takara's microtiter plate by an anti-fibronectin antibody containing an epitope in the CBD region. Termed DOC (Domain Oriented Capture), this solid phase attachment method allows a natural 3-dimensional bFGF structure for enhanced accessibility and binding to heparan sulfate. Detection is by a colorimetric method using biotinylated heparan sulfate as the substrate.

The Peptide Coating Kit allows you to coat plates efficiently with low molecular weight proteins or synthetic peptides that are otherwise difficult to adsorb onto plates.

Back

MK100: Peptide Coating Kit

This product is a tablet for preparing Phosphate Buffered Salts (PBS), which are commonly used for ELISA, Western Blotting and immunohistochemical staining. The buffer can easily be prepared by dissolving the tablet in H₂O. Ten tablets are used to prepare 1,000 ml of PBS (9.57 mM, pH 7.35–7.65).

This product is a tablet for preparing Phosphate Buffered Saline (PBS), which is commonly used for ELISA, Western Blotting and immunohistochemical staining. The buffer can easily be prepared by dissolving the tablet in H₂O. One tablet is used to prepare 1,000 ml of PBS (pH 7.4).

Back

T9181: Phosphate Buffered Saline (PBS) Tablets, pH7.4

This product is a tablet for preparing Phosphate Buffered Saline (PBS), which is commonly used for ELISA, Western Blotting and immunohistochemical staining. The buffer can easily be prepared by dissolving the tablet in H₂O. One tablet is used to prepare 1,000 ml of PBS (pH 7.4) without Potassium. 

Phosphate Buffered Saline with Tween 20 (PBS-T) contains the non-ionic detergent Tween 20 which acts through blocking. This product is a tablet for preparing Phosphate Buffered Saline with Tween 20 (PBS-T), which is commonly used for ELISA and Western Blotting. The buffer can easily be prepared by dissolving the tablet in H₂O. One tablet is used to prepare 1,000 ml of PBS-T (pH 7.4).

Back

T9183: Phosphate Buffered Saline with Tween 20 (PBS-T) Tablets, pH7.4