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Retro-X™ ProteoTuner™ Shield System N (w/ ZsGreen1)

Cat. #

Product

Size

License

Quantity

Details

632167

Retro-X™ ProteoTuner™ Shield System N (w/ ZsGreen1)

Each

USD $740.00

License Statement

ID Number
57	This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The Retro-X ProteoTuner Shield System N (w/ ZsGreen1) uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag on the N-terminus of your protein of interest. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The process of stabilization via Shield1 is reversible, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
635052	Guard1	500 uL	USD $350.00
Guard1 is a ligand that stabilizes proteins tagged with a mutated E. coli DHFR destablilization domain (DD_G). It is used to protect DD_G-tagged proteins from proteasomal degradation. The amount of Guard1 can be varied to fine- tune the amount of your stabilized protein of interest within the cell. Documents Components Image Data Back Rapid and independent control of two proteins Rapid and independent control of two proteins. The ProteoTuner Shield and Guard Systems use separate destabilizing domains and stabilizing ligands. To control two proteins separately but simultaneously, fuse the Shield destabilizing domain (DD) to one protein and the Guard destabilizing domain (DD_G) to the other protein. To stabilize both proteins, add Shield1 and Guard1 simultaneously (left panel). To stabilize just one protein, add either Shield1 or Guard1 (middle panels). To destabilize both proteins, do not add Shield1 or Guard1 (right panel). Back Protein stabilty controlled using the ProteoTuner Guard System Protein stabilty controlled using the ProteoTuner Guard System. AcGFP1 was cloned into the pLVX-PTuner2 Vector downstream of the DD_G and transfected into packaging cells. Viral particles were harvested and used to infect HeLa cells, following standard infection procedures using polybrene and a 1 hr spin. 6 hr post-infection, the media was removed and replaced with new media and cultured for 14 hr. Panel A. The media was removed from the infected HeLa cells and replaced with media containing Guard1 (500 nM). At the indicated timepoints, cells were collected and analyzed via flow cytometry. Panel B. The media was removed from the infected HeLa cells and replaced with media containing different concentrations of Guard1 as indicated. 15 hr later, cells were collected and analyzed via flow cytometry.
632189	Shield1	500 uL	USD $360.00
The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell. Documents Components Image Data Back DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance. While DD-N (solid blue line) is suitable for N-terminal fusions and even C-terminal fusions for many proteins, DD-C (dashed blue line) is optimal for C-terminal fusions and for those proteins that do not tolerate N-terminal fusions. HEK 293 cells expressing the DD fusions noted in the figure legend were treated with varying concentrations of Shield1. 12 hr later, the amount of AcGPF1 stabilized by different concentrations of Shield1 was detected by fluorescence intensity using flow cytometry. DD-C at the C-terminus of AcGFP showed a lower basal level of protein in the absence of Shield1 (purple line; see inset figure). MFI = mean fluorescence intensity. Back Structure of Shield1 stabilization ligand Structure of Shield1 stabilization ligand. Back Ligand-dependent, targeted and reversible protein stabilization Ligand-dependent, targeted and reversible protein stabilization. A small destabilization domain (DD; blue) is fused to a target protein of interest. The small membrane-permeable ligand Shield1 (red) binds to the DD and protects it from proteasomal degradation. Removal of Shield1 however, causes rapid degradation of the entire fusion protein. The default pathway for the ProteoTuner systems is degradation of the fusion protein unless Shield1 is present. Back Protein stabilty controlled using the ProteoTuner shield system Protein stabilty controlled using the ProteoTuner shield system. DsRed-Express was cloned into pRetroX-PTuner IRES and used to infect HeLa cells. The amount of DD-tagged DsRed-Express stabilized by different concentrations of Shield1 was detected via Western blot using the Living Colors DsRed Polyclonal Antibody. Lane 1: molecular weight marker. Lane 2: 1X loading buffer. Lane 3: untreated HeLa cells (no virus, no Shield1). Lane 4: HeLa cells infected with the DD-DsRed Express construct; no Shield1. Lanes 5–8: HeLa cells infected with the DD-DsRed-Express construct and treated with 50, 250, 500, and 1,000 nM Shield1 respectively. Lane 9: 1X loading buffer. Lane 10: HEK 293 DsRed-Express stable cell line. Back 632189: Shield1 Back Control of actin stability with the ProteoTuner system Control of actin stability with the ProteoTuner system. In the absence of Shield1, no DD-AcGFP1 is observed (Panel B) despite the presence of a normal actin filament network (Panel A). In the presence of Shield1, DD-AcGFP1 is stable and present in the actin network (Panel D).

Retro-X™ ProteoTuner™ Shield System N

Cat. #

Product

Size

License

Quantity

Details

632171

Retro-X™ ProteoTuner™ Shield System N

Each

USD $740.00

License Statement

ID Number
57	This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The Retro-X ProteoTuner Shield System N uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag onto the N-terminus of your protein of interest cloned into the pRetroX-PTuner Vector. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The extent of stabilization via Shield1 directly correlates with the amount of the ligand in the medium, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
635052	Guard1	500 uL	USD $350.00
Guard1 is a ligand that stabilizes proteins tagged with a mutated E. coli DHFR destablilization domain (DD_G). It is used to protect DD_G-tagged proteins from proteasomal degradation. The amount of Guard1 can be varied to fine- tune the amount of your stabilized protein of interest within the cell. Documents Components Image Data Back Rapid and independent control of two proteins Rapid and independent control of two proteins. The ProteoTuner Shield and Guard Systems use separate destabilizing domains and stabilizing ligands. To control two proteins separately but simultaneously, fuse the Shield destabilizing domain (DD) to one protein and the Guard destabilizing domain (DD_G) to the other protein. To stabilize both proteins, add Shield1 and Guard1 simultaneously (left panel). To stabilize just one protein, add either Shield1 or Guard1 (middle panels). To destabilize both proteins, do not add Shield1 or Guard1 (right panel). Back Protein stabilty controlled using the ProteoTuner Guard System Protein stabilty controlled using the ProteoTuner Guard System. AcGFP1 was cloned into the pLVX-PTuner2 Vector downstream of the DD_G and transfected into packaging cells. Viral particles were harvested and used to infect HeLa cells, following standard infection procedures using polybrene and a 1 hr spin. 6 hr post-infection, the media was removed and replaced with new media and cultured for 14 hr. Panel A. The media was removed from the infected HeLa cells and replaced with media containing Guard1 (500 nM). At the indicated timepoints, cells were collected and analyzed via flow cytometry. Panel B. The media was removed from the infected HeLa cells and replaced with media containing different concentrations of Guard1 as indicated. 15 hr later, cells were collected and analyzed via flow cytometry.
632189	Shield1	500 uL	USD $360.00
The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell. Documents Components Image Data Back DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance. While DD-N (solid blue line) is suitable for N-terminal fusions and even C-terminal fusions for many proteins, DD-C (dashed blue line) is optimal for C-terminal fusions and for those proteins that do not tolerate N-terminal fusions. HEK 293 cells expressing the DD fusions noted in the figure legend were treated with varying concentrations of Shield1. 12 hr later, the amount of AcGPF1 stabilized by different concentrations of Shield1 was detected by fluorescence intensity using flow cytometry. DD-C at the C-terminus of AcGFP showed a lower basal level of protein in the absence of Shield1 (purple line; see inset figure). MFI = mean fluorescence intensity. Back Structure of Shield1 stabilization ligand Structure of Shield1 stabilization ligand. Back Ligand-dependent, targeted and reversible protein stabilization Ligand-dependent, targeted and reversible protein stabilization. A small destabilization domain (DD; blue) is fused to a target protein of interest. The small membrane-permeable ligand Shield1 (red) binds to the DD and protects it from proteasomal degradation. Removal of Shield1 however, causes rapid degradation of the entire fusion protein. The default pathway for the ProteoTuner systems is degradation of the fusion protein unless Shield1 is present. Back Protein stabilty controlled using the ProteoTuner shield system Protein stabilty controlled using the ProteoTuner shield system. DsRed-Express was cloned into pRetroX-PTuner IRES and used to infect HeLa cells. The amount of DD-tagged DsRed-Express stabilized by different concentrations of Shield1 was detected via Western blot using the Living Colors DsRed Polyclonal Antibody. Lane 1: molecular weight marker. Lane 2: 1X loading buffer. Lane 3: untreated HeLa cells (no virus, no Shield1). Lane 4: HeLa cells infected with the DD-DsRed Express construct; no Shield1. Lanes 5–8: HeLa cells infected with the DD-DsRed-Express construct and treated with 50, 250, 500, and 1,000 nM Shield1 respectively. Lane 9: 1X loading buffer. Lane 10: HEK 293 DsRed-Express stable cell line. Back 632189: Shield1 Back Control of actin stability with the ProteoTuner system Control of actin stability with the ProteoTuner system. In the absence of Shield1, no DD-AcGFP1 is observed (Panel B) despite the presence of a normal actin filament network (Panel A). In the presence of Shield1, DD-AcGFP1 is stable and present in the actin network (Panel D).

pRetroQ-AcGFP1-N1 Vector

Cat. #

Product

Size

License

Quantity

Details

632505

pRetroQ-AcGFP1-N1 Vector

20 ug

USD $568.00

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-AcGFP1-N1 Vector product License Agreement can be found by clicking here.
39	AcGFP is covered by U.S. Patent No. 7,432,053.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-AcGFP1-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the N-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-AcGFP1-C1 Vector

Cat. #

Product

Size

License

Quantity

Details

632506

pRetroQ-AcGFP1-C1 Vector

20 ug

USD $568.00

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-AcGFP1-C1 Vector product License Agreement can be found by clicking here.
39	AcGFP is covered by U.S. Patent No. 7,432,053.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-AcGFP1-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the C-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-DsRed Monomer-N1 Vector

Cat. #

Product

Size

License

Quantity

Details

632507

pRetroQ-DsRed Monomer-N1 Vector

20 ug

USD $568.00

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-DsRed Monomer-N1 Vector product License Agreement can be found by clicking here.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-DsRed Monomer-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the N-terminus of the DsRed-Monomer protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-DsRed Monomer-C1 Vector

Cat. #

Product

Size

License

Quantity

Details

632508

pRetroQ-DsRed Monomer-C1 Vector

20 ug

USD $568.00

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-DsRed Monomer-C1 Vector product License Agreement can be found by clicking here.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-DsRed Monomer-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the C-terminus of the DsRed-monomer protein. Together, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-mCherry-C1 Vector

Cat. #

Product

Size

License

Quantity

Details

632567

pRetroQ-mCherry-C1 Vector

10 ug

USD $568.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-mCherry-C1 Vector product License Agreement can be found by clicking here.

The pRetroQ-mCherry-C1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS downstream of the mCherry coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-mCherry-N1 Vector

Cat. #

Product

Size

License

Quantity

Details

632568

pRetroQ-mCherry-N1 Vector

10 ug

USD $568.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-mCherry-N1 Vector product License Agreement can be found by clicking here.

The pRetroQ-mCherry-N1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroX-IRES-ZsGreen1 Vector

Cat. #	Product	Size		License	Quantity	Details
632520	pRetroX-IRES-ZsGreen1 Vector	20 ug	USD $568.00
The pRetroX-IRES-ZsGreen1 vector is a bicistronic retroviral expression vector containing ZsGreen1 as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and ZsGreen1 to be independently translated from the same mRNA transcript. ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein (ZsGreen) that has been engineered for brighter fluorescence and higher expression in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Mechanism of IRES bicistronic expression from Retro-X retroviral vectors Mechanism of IRES bicistronic expression from Retro-X retroviral vectors. The internal ribosome entry site (IRES) permits a protein of interest and a fluorescent protein to be independently translated from the same mRNA. SD = Splice Donor. SA = Splice Acceptor. ψ = packaging signal. LTR = long terminal repeat. Back Retro-X retroviral vector maps Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP). Back 632520: pRetroX-IRES-ZsGreen1 Vector

pRetroX-IRES-DsredExpress Vector

Cat. #

Product

Size

License

Quantity

Details

632521

pRetroX-IRES-DsredExpress Vector

20 ug

USD $568.00

License Statement

ID Number

72

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pRetroX-IRES-DsredExpress Vector product License Agreement can be found by clicking here.

69

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

The pRetroX-IRES-DsRedExpress vector is a bicistronic retroviral expression vector containing DsRed-Express as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and DsRed-Express to be independently translated from the same mRNA transcript. DsRed-Express is a human codon-optimized variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Retro-X™ ProteoTuner™ Shield System N (w/ ZsGreen1)

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Retro-X™ ProteoTuner™ Shield System N

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pRetroQ-AcGFP1-N1 Vector

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pRetroQ-AcGFP1-C1 Vector

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pRetroQ-DsRed Monomer-N1 Vector

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pRetroQ-DsRed Monomer-C1 Vector

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pRetroQ-mCherry-C1 Vector

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pRetroQ-mCherry-N1 Vector

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pRetroX-IRES-ZsGreen1 Vector

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pRetroX-IRES-DsredExpress Vector

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Rapid and independent control of two proteins

Protein stabilty controlled using the ProteoTuner Guard System

DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance

Structure of Shield1 stabilization ligand

Ligand-dependent, targeted and reversible protein stabilization

Protein stabilty controlled using the ProteoTuner shield system

632189: Shield1

Control of actin stability with the ProteoTuner system

Notice to purchaser

Dose-dependent stabilization of DD-tagged AcGFP1 fluorescent protein

Ligand-dependent, targeted and reversible protein stabilization

Protein stabilty controlled using the ProteoTuner shield system

Rapid and independent control of two proteins

Protein stabilty controlled using the ProteoTuner Guard System

DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance

Structure of Shield1 stabilization ligand

Ligand-dependent, targeted and reversible protein stabilization

Protein stabilty controlled using the ProteoTuner shield system

632189: Shield1

Control of actin stability with the ProteoTuner system

Notice to purchaser

Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions

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Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions

Retroviral vectors available from Clontech

Retro-X retroviral vector maps

632506: pRetroQ-AcGFP1-C1 Vector

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DsRed-Monomer is soluble when expressed in mammalian cells

Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions

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Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions

Retro-X retroviral vector maps

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Retro-X retroviral vector maps

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Retro-X retroviral vector maps

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Mechanism of IRES bicistronic expression from Retro-X retroviral vectors

Retro-X retroviral vector maps

632520: pRetroX-IRES-ZsGreen1 Vector

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Mechanism of IRES bicistronic expression from Retro-X retroviral vectors

Retro-X retroviral vector maps

632521: pRetroX-IRES-DsredExpress Vector

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