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Showing 1-10 of 16 results

Retro-X™ ProteoTuner™ Shield System N (w/ ZsGreen1)

Cat. # Product Size License Quantity Details
632167 Retro-X™ ProteoTuner™ Shield System N (w/ ZsGreen1) Each USD $740.00

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The Retro-X ProteoTuner Shield System N (w/ ZsGreen1) uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag on the N-terminus of your protein of interest. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The process of stabilization via Shield1 is reversible, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
632189 Shield1 500 uL USD $360.00

The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell.

Retro-X™ ProteoTuner™ Shield System N

Cat. # Product Size License Quantity Details
632171 Retro-X™ ProteoTuner™ Shield System N Each USD $740.00

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The Retro-X ProteoTuner Shield System N uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag onto the N-terminus of your protein of interest cloned into the pRetroX-PTuner Vector. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The extent of stabilization via Shield1 directly correlates with the amount of the ligand in the medium, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
632189 Shield1 500 uL USD $360.00

The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell.

pRetroQ-AcGFP1-N1 Vector

Cat. # Product Size License Quantity Details
632505 pRetroQ-AcGFP1-N1 Vector 20 ug USD $568.00

The pRetroQ-AcGFP1-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the N-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroQ-AcGFP1-C1 Vector

Cat. # Product Size License Quantity Details
632506 pRetroQ-AcGFP1-C1 Vector 20 ug USD $568.00

The pRetroQ-AcGFP1-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the C-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroQ-DsRed Monomer-N1 Vector

Cat. # Product Size License Quantity Details
632507 pRetroQ-DsRed Monomer-N1 Vector 20 ug USD $568.00

The pRetroQ-DsRed Monomer-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the N-terminus of the DsRed-Monomer protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroQ-DsRed Monomer-C1 Vector

Cat. # Product Size License Quantity Details
632508 pRetroQ-DsRed Monomer-C1 Vector 20 ug USD $568.00

The pRetroQ-DsRed Monomer-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the C-terminus of the DsRed-monomer protein. Together, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroQ-mCherry-C1 Vector

Cat. # Product Size License Quantity Details
632567 pRetroQ-mCherry-C1 Vector 10 ug USD $568.00

The pRetroQ-mCherry-C1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS downstream of the mCherry coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroQ-mCherry-N1 Vector

Cat. # Product Size License Quantity Details
632568 pRetroQ-mCherry-N1 Vector 10 ug USD $568.00

The pRetroQ-mCherry-N1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroX-IRES-ZsGreen1 Vector

Cat. # Product Size License Quantity Details
632520 pRetroX-IRES-ZsGreen1 Vector 20 ug USD $568.00

The pRetroX-IRES-ZsGreen1 vector is a bicistronic retroviral expression vector containing ZsGreen1 as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and ZsGreen1 to be independently translated from the same mRNA transcript. ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein (ZsGreen) that has been engineered for brighter fluorescence and higher expression in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pRetroX-IRES-DsredExpress Vector

Cat. # Product Size License Quantity Details
632521 pRetroX-IRES-DsredExpress Vector 20 ug USD $568.00

The pRetroX-IRES-DsRedExpress vector is a bicistronic retroviral expression vector containing DsRed-Express as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and DsRed-Express to be independently translated from the same mRNA transcript. DsRed-Express is a human codon-optimized variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 16 results