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Showing 1-10 of 18 results

B. Subtilis Secretory Protein Expression System

Cat. # Product Size License Quantity Details
3380 B. Subtilis Secretory Protein Expression System 10 Rxns USD $645.00

Bacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, the B. subtilis Secretory Protein Expression System from Takara Bio allows rapid development of a library of B. subtilis clones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

HAT™ Protein Expression & Purification System

Cat. # Product Size License Quantity Details
631205 HAT™ Protein Expression & Purification System Each USD $695.00

This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT
sequence and EK site are in frame with the lacZ initiation codon. The vector construct is provided in all three reading frames.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ II

Cat. # Product Size License Quantity Details
3368 SPP System™ II 1 Set USD $844.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ III

Cat. # Product Size License Quantity Details
3369 SPP System™ III 1 Set USD $882.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ IV

Cat. # Product Size License Quantity Details
3370 SPP System™ IV 1 Set USD $844.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊BIC System

Cat. # Product Size License Quantity Details
HB300 ◊BIC System Each USD $1379.00

The Brevibacillus Expression System is an efficient system for producing secreted proteins and is ideal for eukaryotic recombinant protein expression. Brevibacillus choshinensis is a gram-positive bacterium well-suited for heterologous protein expression and is capable of producing high yields of active protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pBIC DNA Set

Cat. # Product Size License Quantity Details
HB310 ◊pBIC DNA Set 1 Kit USD $984.00

The Brevibacillus Expression System is an efficient system for producing secreted proteins and is ideal for eukaryotic recombinant protein expression. Brevibacillus choshinensis is a gram-positive bacterium well-suited for heterologous protein expression and is capable of producing high yields of active protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pET Express & Purify Kit—His60

Cat. # Product Size License Quantity Details
631431 pET Express & Purify Kit—His60 20 Purifications USD $751.00

The pET Express & Purify Kit-His60 allows you to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system includes IPTG-inducible, pET-based bacterial expression vectors that contain a T7/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins. These proteins can be purified with the high-capacity His60 Ni Gravity Columns included in the kit. The kit also contains a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pNC-HisT DNA

Cat. # Product Size License Quantity Details
HB121 ◊pNC-HisT DNA 10 ug USD $414.00

pNC-HisT is a shuttle vector between B. choshinensis and E. coli, as well as a secretory expression vector. Expression plasmids can be constructed by using E. coli as the host, then transferred into B. choshinensis cells. pNC-HisT contains the P2 promoter, which does not work in E. coli but works as a strong promoter in B. choshinensis cells, enabling efficient protein production. A his tag sequence is located downstream of the secretion signal peptide and a thrombin cleavage site is included so that the his tag can be removed from the fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pNC-HisF DNA

Cat. # Product Size License Quantity Details
HB122 ◊pNC-HisF DNA 10 ug USD $414.00

pNC-HisF is a shuttle vector between B. choshinensis and E. coli, as well as a secretory expression vector. Expression plasmids can be constructed by using E. coli as the host, then transferred into B. choshinensis cells. pNC-HisF contains the P2 promoter, which does not work in E. coli but works as a strong promoter in B. choshinensis cells, enabling efficient protein production. A his tag sequence is located downstream of the secretion signal peptide and a Factor Xa cleavage site is included so that the his tag can be removed from the fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 18 results