CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.^＊

＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest.

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Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel

Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel.

CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.^＊

＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Cat. # 3454B contains 3 of Cat. # 3454A. Please refer to Cat. # 3454A for complete product documentation and resources.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed2). rDsRed2 can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed2 expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed2 contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed2, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Monomer) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Monomer contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rDsRed-Monomer, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

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DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A₂₈₀) and chromophore excitation (A₅₅₇) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Express). rDsRed-Express can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed-Express expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Express contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed-Express, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Affinity purified recombinant Aequorea coerulescens green fluorescent protein (rAcGFP1) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rAcGFP1 contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rAcGFP1, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Recombinant green fluorescent protein (rGFP) purified from transformed E. coli using a proprietary method that ensures optimal purity of the GFP protein and integrity of its fluorescence. Suitable for use as a control reagent for GFP expression studies or for microinjection into cells or tissue. Also suitable for use as a standard for protein gels, Western blots, and for fluorometer or FACS machine calibration. GFP is an ~27-kDa monomer with 238 amino acids.

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632373: rGFP Protein

Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. This product is completely chemically defined and free of protein components. Because of this, the Western BLoT Blocking Buffer (Protein Free) provides higher sensitivity than other commonly used blocking agents, such as nonfat milk. In addition to detection of normal proteins, this blocking buffer is excellent for the detection of phosphorylated proteins. In addition, completely protein-free assays are possible when this product is used in combination with Western BLoT Immuno Booster PF (Cat. #T7115A). To obtain the highest detection sensitivity, combine with the Western BLoT HRP Substrate series.

CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes. Cat. # 3453B contains 3 of Cat. # 3453A. Please refer to Cat. # 3453A for complete product documentation and resources.

CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Preheating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes.

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Migration pattern of CLEARLY Protein Ladder (Unstained) (Cat.# 3453A) on a 12% SDS-PAGE gel.

Migration pattern of CLEARLY Protein Ladder (Unstained) (Cat.# 3453A) on a 12% SDS-PAGE gel.