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Showing 1-8 of 8 results
 

pTRE-Cycle2 Vector

Cat. # Product Size License Quantity Details
631116 pTRE-Cycle2 Vector 20 ug USD $561.00

pTRE-Cycle2 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the red fluorescent protein mCherry. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi. Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. mCherry is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pTRE-Cycle3 Vector

Cat. # Product Size License Quantity Details
631117 pTRE-Cycle3 Vector 20 ug USD $561.00

pTRE-Cycle3 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the fluorescent protein ZsGreen1. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi.Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. ZsGreen1 is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pTRE-Cycle1 Vector

Cat. # Product Size License Quantity Details
631115 pTRE-Cycle1 Vector 20 ug USD $541.00

pTRE-Cycle1 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing a second protein that is not cycled. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, PTight-Bi.Expression of the first gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTunerTM destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. The second gene of interest, which is cloned into a separate multiple cloning site, will be coexpressed with the tagged protein, but will not acquire a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Lenti-X™ Packaging Single Shots (Envelope-Free)

Cat. # Product Size License Quantity Details
631294 Lenti-X™ Packaging Single Shots (Envelope-Free) 16 Rxns USD $925.00

Lenti-X Packaging Single Shots (Envelope-Free) enable streamlined production of high-titer lentivirus pseudotyped with the envelope protein of choice by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. The provided pEmpty Vector is used for cloning and expression of any envelope protein of choice (not supplied). Virus production simply involves reconstituting the lyophilized packaging mix with a lentiviral transfer vector of choice together with a plasmid encoding for the desired protein envelope in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. # 632180), in a 10-cm dish.

Cat. # 631294 consists of 16 tubes of Lenti-X Packaging Single Shots (Envelope-Free; Cat. # 631297), the pEmpty Vector (Cat. # 631298) for cloning and expression of the desired envelope protein, and the Lenti-X Reporter Vector Set (Cat. # 632677), which includes the self-inactivating transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Lenti-X™ SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Full Length)

Cat. # Product Size License Quantity Details
631292 Lenti-X™ SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Full Length) 12 Rxns USD $1024.00

Lenti-X SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Full Length) enable streamlined production of high-titer lentivirus pseudotyped with the spike protein from SARS-CoV-2 (B.1.351 variant) by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involves reconstituting a tube of lyophilized packaging mix with a lentiviral transfer vector of choice in sterile water and adding the mixture to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.

Each kit includes 12 tubes of Lenti-X SARS-CoV-2 Packaging Mix (B.1.351 Spike, Full Length; Cat. # 631290), 6 tubes of Lenti-X SARS-CoV-2 Packaging Mix (No-Envelope Control; Cat. # 632676) for production of pseudoviral particles lacking an envelope protein, and the Lenti-X SARS-CoV-2 Vector Set (Cat. # 632677), which includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.

For your convenience, the packaging mixes included in the kit are also sold separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Lenti-X™ SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Truncated)

Cat. # Product Size License Quantity Details
631293 Lenti-X™ SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Truncated) 12 Rxns USD $1024.00

Lenti-X SARS-CoV-2 Packaging Single Shots (B.1.351 Spike, Truncated) enable streamlined production of high-titer lentivirus pseudotyped with the spike protein from SARS-CoV-2 (B.1.351 variant) by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involves reconstituting a tube of lyophilized packaging mix with a lentiviral transfer vector of choice in sterile water and adding the mixture to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.

Each kit includes 12 tubes of Lenti-X SARS-CoV-2 Packaging Mix (B.1.351 Spike, Truncated; Cat. # 631291), 6 tubes of Lenti-X SARS-CoV-2 Packaging Mix (No-Envelope Control; Cat. # 632676) for production of pseudoviral particles lacking an envelope protein, and the Lenti-X SARS-CoV-2 Vector Set (Cat. # 632677), which includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.

For your convenience, the packaging mixes included in the kit are also sold separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Lenti-X™ SARS-CoV-2 Packaging Single Shots (Universal)

Cat. # Product Size License Quantity Details
631295 Lenti-X™ SARS-CoV-2 Packaging Single Shots (Universal) 16 Rxns USD $1095.00

Lenti-X SARS-CoV-2 Packaging Single Shots (Universal) allow streamlined production of high-titer lentivirus pseudotyped with your SARS-CoV-2 spike protein of interest by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involved diluting a SARS-CoV-2 spike variant expression vector and your transfer vector of choice in water, adding to a tube of single shots, and applying the mixture to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.

Each kit includes 16 tubes of Lenti-X Packaging Single Shots (Envelope-Free; Cat. # 631297), the pEmpty Vector (Cat. # 631298) for cloning and expression of the desired SARS-CoV-2 spike protein, the Lenti-X SARS-CoV-2 Spike Vector Set (Cat. # 631296), which includes three plasmids encoding for truncated SARS-CoV-2 spike variants D614G, B.1.1.7, and B.1.351 and the Lenti-X Reporter Vector Set (Cat. # 632677). Cat. # 632677 includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

rLV.EF1.Tet3G-9

Cat. # Product Size License Quantity Details
0055VCT rLV.EF1.Tet3G-9 1 x 20 uL USD $714.00

Highly concentrated and purified lentivirus for delivery of the Tet-On 3G transactivator to target cells.  Any gene of interest under the control of a TRE3G promoter can be tightly controlled by the presence and absence of Dox in the culture medium. Tet-On 3G is a modified, improved Tet-On Advanced transcriptional activator protein (transactivator) which has been evolved to display far higher sensitivity to the inducer molecule dox. Tet-On 3G generates very high maximal expression and responds to lower Dox concentrations than its predecessors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Showing 1-8 of 8 results