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Reef Coral Fluorescent Protein Vector Set

Cat. #	Product	Size		License	Quantity	Details
630050	Reef Coral Fluorescent Protein Vector Set	Each	USD $5444.00
A set of twelve vectors encoding novel fluorescent proteins in four colors: cyan, green, red and yellow. Four constructs are prokaryotic expression vectors that also serve as convenient sources of fluorescent protein cDNAs. In each of these prokaryotic vectors, the full-length cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. Eight of the vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion to any of the four fluorescent proteins. The fusion vectors can also be used as cotransfection markers; the unmodified vectors will express fluorescent protein. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like

rDsRed2 Protein

Cat. #	Product	Size		License	Quantity	Details
632436	rDsRed2 Protein	100 ug	USD $422.00
Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed2). rDsRed2 can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed2 expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed2 contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed2, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like

rDsRed-Monomer Protein

Cat. #

Product

Size

License

Quantity

Details

632503

rDsRed-Monomer Protein

100 ug

USD $422.00

License Statement

ID Number
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Monomer) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Monomer contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rDsRed-Monomer, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

rDsRed-Express Protein

Cat. #

Product

Size

License

Quantity

Details

632437

rDsRed-Express Protein

100 ug

USD $422.00

License Statement

ID Number
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Express). rDsRed-Express can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed-Express expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Express contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed-Express, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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rAcGFP1 Protein

Cat. #

Product

Size

License

Quantity

Details

632502

rAcGFP1 Protein

100 ug

USD $422.00

License Statement

ID Number
39	AcGFP is covered by U.S. Patent No. 7,432,053.

Affinity purified recombinant Aequorea coerulescens green fluorescent protein (rAcGFP1) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rAcGFP1 contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rAcGFP1, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

rGFP Protein

Cat. #	Product	Size		License	Quantity	Details
632373	rGFP Protein	100 ug	USD $422.00
Recombinant green fluorescent protein (rGFP) purified from transformed E. coli using a proprietary method that ensures optimal purity of the GFP protein and integrity of its fluorescence. Suitable for use as a control reagent for GFP expression studies or for microinjection into cells or tissue. Also suitable for use as a standard for protein gels, Western blots, and for fluorometer or FACS machine calibration. GFP is an ~27-kDa monomer with 238 amino acids. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 632373: rGFP Protein

pAcGFP1-Endo Vector

Cat. #

Product

Size

License

Quantity

Details

632490

pAcGFP1-Endo Vector

20 ug

USD $589.00

License Statement

ID Number

72

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pAcGFP1-Endo Vector product License Agreement can be found by clicking here.

39

AcGFP is covered by U.S. Patent No. 7,432,053.

pAcGFP1-Endo encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein, c-Myc, and RhoB protein. The RhoB GTPase localizes the fusion protein to vesicles involved in endocytosis and allows monitoring of intracellular membrane traffic in living and fixed cells using fluorescence microscopy. The c-Myc epitope allows detection of the fusion protein using c-Myc antibodies. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pTRE-Cycle2 Vector

Cat. #

Product

Size

License

Quantity

Details

631116

pTRE-Cycle2 Vector

20 ug

USD $561.00

License Statement

ID Number
57	This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pTRE-Cycle2 Vector product License Agreement can be found by clicking here.

pTRE-Cycle2 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the red fluorescent protein mCherry. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, P_Tight-Bi. Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. mCherry is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pTRE-Cycle3 Vector

Cat. #

Product

Size

License

Quantity

Details

631117

pTRE-Cycle3 Vector

20 ug

USD $561.00

License Statement

ID Number

42

Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research

57

This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.

pTRE-Cycle3 is a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate (i.e., cycle) the amount of a protein of interest in mammalian cells while coexpressing the fluorescent protein ZsGreen1. Expression of both proteins is tightly regulated by the bidirectional, TRE-based promoter, P_Tight-Bi.Expression of the gene of interest is controlled by an additional mechanism, since the resulting protein is fused to an N-terminal ProteoTuner destabilization domain (DD), which causes rapid proteasomal degradation of any protein to which it is fused. The degradation process can be reversed repeatedly by adding the stabilizing ligand Shield1 and then removing it from the culture medium. ZsGreen1 is coexpressed with the tagged protein, but does not contain a DD-tag, and thus is subject only to Tet-based regulation. The vector is intended for use with any Tet-On or Tet-Off Advanced Expression System. To select stable cell lines, the vector must be cotransfected with one of the provided linear selection markers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pLVX-DsRed-Express2-C1 Vector

Cat. #

Product

Size

License

Quantity

Details

632559

pLVX-DsRed-Express2-C1 Vector

10 ug

USD $676.00

License Statement

ID Number
55	cPPT Element. This product and its use are the subject to one or more of the following U.S. Pat. No. 8,093,042. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA, Inc. is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pLVX-DsRed-Express2-C1 Vector product License Agreement can be found by clicking here.
K11	This product and its use are the subject to one or more of the following U.S. Pat. 8,093,042 and foreign equivalents. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

This lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression in mammalian cells. Inserting a cDNA in the MCS downstream of the DsRed-Express2 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots (VSV-G) and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Reef Coral Fluorescent Protein Vector Set

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rDsRed2 Protein

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rDsRed-Monomer Protein

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rDsRed-Express Protein

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rAcGFP1 Protein

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rGFP Protein

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pAcGFP1-Endo Vector

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pTRE-Cycle2 Vector

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pTRE-Cycle3 Vector

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pLVX-DsRed-Express2-C1 Vector

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is a monomeric protein

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

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632373: rGFP Protein

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AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

632490: pAcGFP1-Endo Vector

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Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline

Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins and also for Sox2 and Oct4

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Inducible expression of two proteins of interest, one with a DD tag and one induced by tetracycline

Independent inducible expression of two proteins in HEK 293 cells is shown for red and green fluorescent proteins and also for Sox2 and Oct4

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Robust expression of DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells

Lentiviral vectors with fluorescent proteins

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