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Showing 1-9 of 9 results
 

Recombinant Cas9 Protein GMP grade

Cat. # Product Size License Quantity Details
T230 Recombinant Cas9 Protein GMP grade 0.6 mg USD $2998.00

Recombinant Cas9 Protein GMP grade, is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease with a nuclear localization signal (NLS) located at the C-terminus of the protein. It is manufactured and tested in accordance with the following guidelines: "Standards for Manufacturing Control and Quality Control, etc. of Investigational Products (Investigational products GMP)," as notified by the Ministry of Health and Welfare Japan ("PFSB Notification No. 0709002, July 9, 2008"; PFSB = Pharmaceutical and Food Safety Bureau). No animal- or human-derived components are used in the manufacture of this product. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single-guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ Recombinant Cas9 (10 µg/µl)

Cat. # Product Size License Quantity Details
632678 Guide-it™ Recombinant Cas9 (10 µg/µl) 200 ug USD $389.00

Guide-it Recombinant Cas9 (10 µg/µl) is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a C-terminal nuclear localization signal (NLS), provided in 50% glycerol. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ Recombinant Cas9 (10 µg/µl)

Cat. # Product Size License Quantity Details
632679 Guide-it™ Recombinant Cas9 (10 µg/µl) 500 ug USD $725.00

Guide-it Recombinant Cas9 (10 µg/µl) is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a C-terminal nuclear localization signal (NLS), provided in 50% glycerol. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Gesicle Producer 293T Cell Line

Cat. # Product Size License Quantity Details
632617 Gesicle Producer 293T Cell Line 1 mL USD $361.00

The Gesicle Producer 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. Gesicles are produced in these cells via co-overexpression of packaging mix components, which include a nanovesicle-inducing glycoprotein and a protein that is displayed on the cell surface and mediates binding and fusion with the cellular membrane of target cells. Simultaneous overexpression of another protein cargo can result in incorporation of that protein within the gesicles. When combined with a gesicle production system, these cells are capable of producing high gesicle yields.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ CRISPR/Cas9 Gesicle Production System

Cat. # Product Size License Quantity Details
632613 Guide-it™ CRISPR/Cas9 Gesicle Production System 1 System USD $713.00

The Guide-it CRISPR/Cas9 Gesicle Production System is a system for producing high yields of target-specific CRISPR/Cas9 gesicles for gene editing. Gesicles are cell-derived nanovesicles used to deliver macromolecular cargoes to a broad range of target cells, including cells that are difficult to transfect with plasmids. The nanovesicles are produced in a Gesicle Producer 293T Cell Line (Cat. No. 632617) via co-overexpression of packaging mix components, which include a nanovesicle-inducing glycoprotein and a protein that is displayed on the cell surface that mediates binding and fusion with the cellular membrane of target cells. Simultaneous overexpression of additional macromolecular cargoes, in this case the Cas9 protein from Streptococcus pyogenes and a target-specific guide RNA (sgRNA), results in incorporation of the Cas9/sgRNA complex within the gesicles. After the resulting Cas9/sgRNA gesicles are harvested and applied to your target cells in the presence of protamine sulfate, they will efficiently enter the cells and mediate gene editing. This system provides the components needed to clone and express your target-specific guide RNA, and packaging reagents to produce CRISPR/Cas9 gesicles. It is essential to use the pGuide-it-sgRNA1 Vector for expression and packaging of your guide RNA, since using other commonly used guide RNA vectors will not result in effective Cas9/sgRNA complexes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ CRISPR/Cas9 Gesicle Packaging Set

Cat. # Product Size License Quantity Details
632616 Guide-it™ CRISPR/Cas9 Gesicle Packaging Set 10 Rxns USD $610.00

Gesicles are cell-derived nanovesicles used to deliver macromolecular cargoes to a broad range of target cells, including cells that are difficult to transfect with plasmids. The nanovesicles generated using the Guide-it CRISPR/Cas9 Gesicle Packaging Set are produced in a Gesicle Producer 293T Cell Line (Cat. No. 632617) via co-overexpression of packaging mix components, which include a nanovesicle-inducing glycoprotein and a protein that is displayed on the cell surface and mediates binding and fusion with the cellular membrane of target cells. Simultaneous overexpression of additional macromolecular cargoes, in this case the Cas9 protein from Streptococcus pyogenes and a target-specific guide RNA (sgRNA), results in incorporation of the Cas9/sgRNA complex within the gesicles. After the resulting Cas9/sgRNA gesicles are harvested and applied to your target cells in the presence of protamine sulfate, they will efficiently enter the cells and mediate gene editing. The Guide-it CRISPR/Cas9 Gesicles Packaging Mixes 1 and 2 are each supplied prealiquoted and lyophilized in single vials containing Xfect Transfection Reagent and an optimized combination of gesicle-producing components. Gesicles are produced by sequentially reconstituting the lyophilized mixtures with your guide RNA vector of choice in sterile water and transfecting the sample into Gesicle Producer 293T cells according to the protocol in the Guide-it CRISPR/Cas9 Gesicle Production System User Manual. It is essential to use the pGuide-itsgRNA1 Vector for expression/packaging of your guide RNA, since using other commonly used guide RNA vectors will not result in effective Cas9/sgRNA complexes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Lenti-X™ Tet-On® 3G CRISPR/Cas9 System

Cat. # Product Size License Quantity Details
632633 Lenti-X™ Tet-On® 3G CRISPR/Cas9 System 1 System USD $1768.00

The Lenti-X Tet-On 3G CRISPR/Cas9 System is designed for Tet-inducible Cas9 expression along with expression of a custom sgRNA. Because Cas9 is only expressed in the presence of doxycycline, genome editing is achieved on demand and without the side effects of persistent Cas9 expression in cell culture. This kit contains all of the components necessary for generating three types of lentivirus: one for constitutive expression of the Tet-On 3G transactivator protein, a second for Tet-inducible Cas9 expression under the PTRE3GV promoter, and a third for constitutive sgRNA expression under the U6 promoter

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ CRISPR/Cas9 System (Green)

Cat. # Product Size License Quantity Details
632601 Guide-it™ CRISPR/Cas9 System (Green) 1 System USD $325.00

The Guide-it CRISPR/Cas9 System (Green) is a complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-ZsGreen1 Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein ZsGreen1. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and ZsGreen1 are coexpressed from a bidirectional CMV promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Guide-it™ CRISPR/Cas9 System (Red)

Cat. # Product Size License Quantity Details
632602 Guide-it™ CRISPR/Cas9 System (Red) 1 System USD $325.00

The Guide-it CRISPR/Cas9 System (Red) is a complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-tdTomato Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein tdTomato. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and tdTomato are coexpressed from a bidirectional CMV promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-9 of 9 results