Refine Your Search

Available results will be divided among three tabs below.
Options for narrowing the number of matches are available on the left.

Adding terms to the "Refine Your Search" box will apply more criteria to your current results. Making selections in the "Search Filters" box will further limit your results within a variety of categories, such as application, product type, kit size, etc.

Showing 1-10 of 54 results

B. Subtilis Secretory Protein Expression System

Cat. # Product Size License Quantity Details
3380 B. Subtilis Secretory Protein Expression System 10 Rxns USD $645.00

Bacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, the B. subtilis Secretory Protein Expression System from Takara Bio allows rapid development of a library of B. subtilis clones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Human Cell-Free Protein Expression System

Cat. # Product Size License Quantity Details
3281 Human Cell-Free Protein Expression System 10 Rxns USD $364.00

The Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

HAT™ Protein Expression & Purification System

Cat. # Product Size License Quantity Details
631205 HAT™ Protein Expression & Purification System Each USD $695.00

This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT
sequence and EK site are in frame with the lacZ initiation codon. The vector construct is provided in all three reading frames.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ II

Cat. # Product Size License Quantity Details
3368 SPP System™ II 1 Set USD $844.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ III

Cat. # Product Size License Quantity Details
3369 SPP System™ III 1 Set USD $882.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SPP System™ IV

Cat. # Product Size License Quantity Details
3370 SPP System™ IV 1 Set USD $844.00

The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.

Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ ProS2 DNA

Cat. # Product Size License Quantity Details
3371 ◊pCold™ ProS2 DNA 25 ug USD $745.00

Takara's pCold ProS2 DNA is a cold shock expression vector that includes Protein S derived from a Gram-negative myxobacteria Myxococcus xanthus as a solubilization tag. Protein S, (173 aa) is very stable soluble protein present in the spore surface coat of Myxococcus xanthus. Fusing a target gene encoding a protein of interest with the ProS2 Tag, found in pCold ProS2 DNA may induce the stability and solubility of the fusion protein.

The pCold ProS2 DNA Vector consists of the cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation
enhancing element (TEE), a His-Tag sequence, ProS2 Tag, and a multicloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure
strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between ProS2 Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein. Most E. coli strains can serve as expression hosts. The pCold ProS2 DNA Vector provides cold shock technology for high yield protein expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ Vector Set

Cat. # Product Size License Quantity Details
3360 ◊pCold™ Vector Set 1 Set USD $1159.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ I DNA

Cat. # Product Size License Quantity Details
3361 ◊pCold™ I DNA 25 ug USD $435.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors (including pCold I) contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). pCold I also contains a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ II DNA

Cat. # Product Size License Quantity Details
3362 ◊pCold™ II DNA 25 ug USD $441.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 54 results