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Showing 1-10 of 92 results

HAT™ Protein Expression & Purification System

Cat. # Product Size License Quantity Details
631205 HAT™ Protein Expression & Purification System Each USD $695.00

This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT
sequence and EK site are in frame with the lacZ initiation codon. The vector construct is provided in all three reading frames.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ ProS2 DNA

Cat. # Product Size License Quantity Details
3371 ◊pCold™ ProS2 DNA 25 ug USD $745.00

Takara's pCold ProS2 DNA is a cold shock expression vector that includes Protein S derived from a Gram-negative myxobacteria Myxococcus xanthus as a solubilization tag. Protein S, (173 aa) is very stable soluble protein present in the spore surface coat of Myxococcus xanthus. Fusing a target gene encoding a protein of interest with the ProS2 Tag, found in pCold ProS2 DNA may induce the stability and solubility of the fusion protein.

The pCold ProS2 DNA Vector consists of the cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation
enhancing element (TEE), a His-Tag sequence, ProS2 Tag, and a multicloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure
strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between ProS2 Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein. Most E. coli strains can serve as expression hosts. The pCold ProS2 DNA Vector provides cold shock technology for high yield protein expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

HRV 3C Protease

Cat. # Product Size License Quantity Details
7360 HRV 3C Protease 500 Units USD $283.00

HRV 3C Protease is a recombinant 3C protease derived from human Rhinovirus type 14 expressed in E. coli. This product is a highly purified 6xHN-tagged protein. The enzyme has the same activity as the native protein and cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro). It is useful for cleaving tag sequences from fusion proteins that contain a HRV 3C Protease cleavage site. Because this product has a N-terminal 6xHN tag (which is a modified His-tag), it can be easily eliminated from the protease reaction solution through immobilized metal affinity chromatography (IMAC) using TALON Metal Affinity Resin or His60 Ni Superflow Resin. Additionally, because the enzyme is active at 4℃, protein cleavage reactions can be performed under conditions that do not affect the activity or stability of the target protein. This product includes a Cleavage Control Fusion Protein. The Control Protein has a N-terminal 6xHN tag. Digestion of the Control Protein with HRV 3C Protease results in a 52 kDa TF protein and a 24 kDa GST peptide. Successful cleavage of the Control Protein can be easily confirmed using SDS-PAGE.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ Vector Set

Cat. # Product Size License Quantity Details
3360 ◊pCold™ Vector Set 1 Set USD $1159.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ I DNA

Cat. # Product Size License Quantity Details
3361 ◊pCold™ I DNA 25 ug USD $435.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors (including pCold I) contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). pCold I also contains a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ II DNA

Cat. # Product Size License Quantity Details
3362 ◊pCold™ II DNA 25 ug USD $441.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pT7-IRES His-N DNA

Cat. # Product Size License Quantity Details
3290 pT7-IRES His-N DNA 20 ug USD $219.00

pT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES His-N DNA is an expression vector including a his-tag sequence. Using pT7-IRES His-N DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as an N-terminal fusion with a his tag. A Factor Xa cleavage site is included so that the his tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pT7-IRES His-C DNA

Cat. # Product Size License Quantity Details
3291 pT7-IRES His-C DNA 20 ug USD $219.00

pT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES His-C DNA is an expression vector including a his-tag sequence. Using pT7-IRES His-C DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as a C-terminal fusion with a his tag. A Factor Xa cleavage site is included so that the his tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pT7-IRES Myc-N DNA

Cat. # Product Size License Quantity Details
3292 pT7-IRES Myc-N DNA 20 ug USD $219.00

pT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES Myc-N DNA is an expression vector including a c-Myc tag sequence. Using pT7-IRES Myc-N DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as an N-terminal fusion with a c-Myc tag. A Factor Xa cleavage site is included so that the c-Myc tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ TF DNA

Cat. # Product Size License Quantity Details
3365 ◊pCold™ TF DNA 25 ug USD $799.00

The pCold TF DNA Vector provides cold shock technology for high yield protein expression combined with Trigger Factor (chaperone) expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins. pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNA Vector consists of the cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a His-Tag sequence, and a multicloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between TF-Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein. E. coli strains can serve as expression hosts.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 92 results