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Showing 1-10 of 72 results

◊pCold™ ProS2 DNA

Cat. # Product Size License Quantity Details
3371 ◊pCold™ ProS2 DNA 25 ug USD $745.00

Takara's pCold ProS2 DNA is a cold shock expression vector that includes Protein S derived from a Gram-negative myxobacteria Myxococcus xanthus as a solubilization tag. Protein S, (173 aa) is very stable soluble protein present in the spore surface coat of Myxococcus xanthus. Fusing a target gene encoding a protein of interest with the ProS2 Tag, found in pCold ProS2 DNA may induce the stability and solubility of the fusion protein.

The pCold ProS2 DNA Vector consists of the cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation
enhancing element (TEE), a His-Tag sequence, ProS2 Tag, and a multicloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure
strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between ProS2 Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein. Most E. coli strains can serve as expression hosts. The pCold ProS2 DNA Vector provides cold shock technology for high yield protein expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

HRV 3C Protease

Cat. # Product Size License Quantity Details
7360 HRV 3C Protease 500 Units USD $283.00

HRV 3C Protease is a recombinant 3C protease derived from human Rhinovirus type 14 expressed in E. coli. This product is a highly purified 6xHN-tagged protein. The enzyme has the same activity as the native protein and cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro). It is useful for cleaving tag sequences from fusion proteins that contain a HRV 3C Protease cleavage site. Because this product has a N-terminal 6xHN tag (which is a modified His-tag), it can be easily eliminated from the protease reaction solution through immobilized metal affinity chromatography (IMAC) using TALON Metal Affinity Resin or His60 Ni Superflow Resin. Additionally, because the enzyme is active at 4℃, protein cleavage reactions can be performed under conditions that do not affect the activity or stability of the target protein. This product includes a Cleavage Control Fusion Protein. The Control Protein has a N-terminal 6xHN tag. Digestion of the Control Protein with HRV 3C Protease results in a 52 kDa TF protein and a 24 kDa GST peptide. Successful cleavage of the Control Protein can be easily confirmed using SDS-PAGE.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ Vector Set

Cat. # Product Size License Quantity Details
3360 ◊pCold™ Vector Set 1 Set USD $1159.00

Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ I DNA

Cat. # Product Size License Quantity Details
3361 ◊pCold™ I DNA 25 ug USD $435.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors (including pCold I) contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). pCold I also contains a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ II DNA

Cat. # Product Size License Quantity Details
3362 ◊pCold™ II DNA 25 ug USD $441.00

Takara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat.# 3340).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ TF DNA

Cat. # Product Size License Quantity Details
3365 ◊pCold™ TF DNA 25 ug USD $799.00

The pCold TF DNA Vector provides cold shock technology for high yield protein expression combined with Trigger Factor (chaperone) expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins. pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNA Vector consists of the cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a His-Tag sequence, and a multicloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between TF-Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein. E. coli strains can serve as expression hosts.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

◊pCold™ GST DNA

Cat. # Product Size License Quantity Details
3372 ◊pCold™ GST DNA 25 ug USD $476.00

The expression vector pCold GST DNA was developed by incorporating glutathione S-transferase (GST) derived from Schistosoma japonicum as a soluble tag. The proteins expressed using this vector have an N-terminal GST tag, which can improve the stability and solubility of the fused protein. The pCold GST vector includes a 5' untranslated region (5' UTR), translation enhancing element (TEE), his tag, GST tag, and multiple cloning site (MCS) downstream of the cspA promoter. In addition, a lac operator has been inserted downstream of the promoter to allow precise control of gene expression. Finally, because the pCold vector series uses an E. coli promoter, virtually any strain of E. coli can be used as a host for protein expression.

High affinity purification of the GST-tagged fusion proteins expressed using pCold GST is possible. Furthermore, a highly specific HRV 3C protease recognition sequence has been inserted between the GST tag and MCS, allowing removal of the GST tag from the recombinant protein. Because the optimum reaction temperature for HRV 3C protease is low (4–5℃), tag cleavage can be performed under moderate conditions.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Capturem™ His-Tagged Purification Large Volume

Cat. # Product Size License Quantity Details
635724 Capturem™ His-Tagged Purification Large Volume 4 Units (2 Units per box) USD $460.00

The Capturem His-Tagged Purification Large Volume unit lets you quickly purify up to 25 mg of protein (depending on protein size and expression level) from a large volume of lysate (up to 500 ml at a time), resulting in purified protein concentrated at up to 2 mg/ml. Like our other his-tagged Capturem products, the Large Volume unit works for bacterial and mammalian expression systems with a wide range of additives. Each unit requires a minimum elution volume of 1.5 ml.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pET Express & Purify Kit—HisTALON™

Cat. # Product Size License Quantity Details
631430 pET Express & Purify Kit—HisTALON™ 20 Purifications USD $786.00

The pET Express & Purify Kit-HisTALON allows you to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system includes IPTG-inducible, pET-based bacterial expression vectors that contain a T7/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins. These proteins can be made exceptionally pure with the high-affinity HisTALON Gravity Columns included in the kit. The kit also includes a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

pET Express & Purify Kit—HisTALON™ (In-Fusion® Ready)

Cat. # Product Size License Quantity Details
631429 pET Express & Purify Kit—HisTALON™ (In-Fusion® Ready) 20 Purifications USD $821.00

The pET Express & Purify Kit-HisTALON (In-Fusion Ready) allows you to use In-Fusion cloning technology to express a protein of interest with an N- or C-terminal 6xHN tag in E. coli. The system contains IPTG-inducible, pET-based bacterial expression vectors that have been prelinearized to allow In-Fusion cloning via the included In-Fusion HD Enzyme Premix. These vectors contain a T7/lac promoter and polyhistidine tag sequence for high-level expression of his-tagged proteins, which can be made exceptionally pure with the high-affinity HisTALON Gravity Columns included in the kit. The kit also contains a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 72 results