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Showing 1-10 of 283 results

B. Subtilis Secretory Protein Expression System

Cat. # Product Size License Quantity Details
3380 B. Subtilis Secretory Protein Expression System 10 Rxns USD $645.00

Bacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, the B. subtilis Secretory Protein Expression System from Takara Bio allows rapid development of a library of B. subtilis clones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.

NOTE: For commercial use of this product, please fill out the commercial use inquiry form. Upon submission of the form, you will be directed to a page with appropriate ordering information.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Human Cell-Free Protein Expression System

Cat. # Product Size License Quantity Details
3281 Human Cell-Free Protein Expression System 10 Rxns USD $364.00

The Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Capturem™ Protein A Buffer

Cat. # Product Size License Quantity Details
635746 Capturem™ Protein A Buffer 100 mL USD $78.00

Capturem Protein A Buffer is used for purification of IgGs on Capturem Protein A Miniprep Columns (Catalog # 635717), Capturem Protein A 96-Well Plate (Catalog # 635716), Capturem Protein A 24-Well Plate - HC (Catalog # 635743), and Capturem Protein A Maxiprep Columns (Catalog # 635720).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

TaKaRa® CBB Protein Safe Stain

Cat. # Product Size License Quantity Details
T9320A TaKaRa® CBB Protein Safe Stain 1 L USD $140.00

Takara CBB Protein Safe Stain is a highly sensitive protein staining reagent based on Coomassie Brilliant Blue G-250. This product can be used to stain SDS-PAGE or native PAGE gels quickly. It allows the visualization of protein bands approximately 5 minutes after the staining process begins, and provides maximum sensitivity after 60 minutes of staining. Stained gels may be destained with deionized water to remove background staining. Takara CBB Protein Safe Stain can detect bands containing as little as 8 ng of protein. It can be handled safely, since it does not contain methanol or acetic acid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

CLEARLY Stained Protein Ladder

Cat. # Product Size License Quantity Details
3454A CLEARLY Stained Protein Ladder 500 uL USD $156.00

CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.

*: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

CLEARLY Stained Protein Ladder

Cat. # Product Size License Quantity Details
3454B CLEARLY Stained Protein Ladder 1,500 uL USD $442.00

CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.

*: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Cat. # 3454B contains 3 of Cat. # 3454A. Please refer to Cat. # 3454A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Western BLoT Blocking Buffer (Protein Free)

Cat. # Product Size License Quantity Details
T7132A Western BLoT Blocking Buffer (Protein Free) 500 mL USD $167.00

Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. This product is completely chemically defined and free of protein components. Because of this, the Western BLoT Blocking Buffer (Protein Free) provides higher sensitivity than other commonly used blocking agents, such as nonfat milk. In addition to detection of normal proteins, this blocking buffer is excellent for the detection of phosphorylated proteins. In addition, completely protein-free assays are possible when this product is used in combination with Western BLoT Immuno Booster PF (Cat. #T7115A). To obtain the highest detection sensitivity, combine with the Western BLoT HRP Substrate series.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

CLEARLY Protein Ladder (Unstained)

Cat. # Product Size License Quantity Details
3453A CLEARLY Protein Ladder (Unstained) 500 uL USD $156.00

CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Preheating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

CLEARLY Protein Ladder (Unstained)

Cat. # Product Size License Quantity Details
3453B CLEARLY Protein Ladder (Unstained) 1,500 uL USD $442.00

CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes. Cat. # 3453B contains 3 of Cat. # 3453A. Please refer to Cat. # 3453A for complete product documentation and resources.


Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Protein Molecular Weight Marker (High)

Cat. # Product Size License Quantity Details
3451 Protein Molecular Weight Marker (High) 200 Lanes USD $167.00

Takara's Protein Molecular Weight Markers are designed for use in SDS Polyacrylamide gel electrophoresis. These markers come in three ranges: Low (Molecular weight range: 14.3–97.2 kDa), High (Molecular weight range: 44.3–200 kDa) and Broad (Molecular weight range: 6.5–200 kDa). Each protein in these markers is quantitated to yield uniform band intensities when stained with Coomassie Brilliant Blue R-250 after running on an SDS polyacrylamide gel. Five μl of 20-fold diluted marker is recommended per lane of SDS-PAGE minigel for standard use.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 283 results