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Showing 1-10 of 1247 results

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Human Cell-Free Protein Expression System and vectors

The Human Cell-Free Protein Expression System provides in vitro transcription and translation in a single step with high levels of protein expression.

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Protein transfection reagents

Peptide-based transfection reagent that efficiently delivers a large amount of biologically active protein directly into live cells.

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Rapid, complete protein digestion for mass spectrometry—Capturem Trypsin and Capturem Pepsin

Membrane-immobilized trypsin or pepsin in a mini spin column format for rapid, complete protein digestion.

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GST-tagged protein purification kit

GST-tagged protein purification elutes highly purified protein free of contaminants, and requires no optimization.

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Cell lysis buffer—reagent for protein extraction

Get a flexible, efficient cell lysis buffer for protein extraction from a wide variety of cells. This cell lysis reagent offers a 10-min lysis using mild, non-denaturing conditions.

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NucleoSpin RNA/Protein—isolation and purification of RNA and protein from ONE lysate

Rapid, high-yield co-purification of total RNA and protein from cells and tissue.

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Myc-tagged protein purification

Monoclonal c-Myc antibody crosslinked to immobilized Protein A agarose beads.

B. Subtilis Secretory Protein Expression System

Cat. # Product Size Price License Quantity Details
3380 B. Subtilis Secretory Protein Expression System 10 Rxns $614.00

Bacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, the B. subtilis Secretory Protein Expression System from Takara Bio allows rapid development of a library of B. subtilis clones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify the signal
peptide that results in efficient expression of your desired secreted protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Human Cell-Free Protein Expression System

Cat. # Product Size Price License Quantity Details
3281 Human Cell-Free Protein Expression System 10 Rxns $336.00

The Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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TaKaRa® CBB Protein Safe Stain

Cat. # Product Size Price License Quantity Details
T9320A TaKaRa® CBB Protein Safe Stain 1 L $125.00

Takara CBB Protein Safe Stain is a highly sensitive protein staining reagent based on Coomassie Brilliant Blue G-250. This product can be used to stain SDS-PAGE or native PAGE gels quickly. It allows the visualization of protein bands approximately 5 minutes after the staining process begins, and provides maximum sensitivity after 60 minutes of staining. Stained gels may be destained with deionized water to remove background staining. Takara CBB Protein Safe Stain can detect bands containing as little as 8 ng of protein. It can be handled safely, since it does not contain methanol or acetic acid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Showing 1-10 of 1247 results