Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R² values are shown in the graph; Nextera XT R²=0.9961, PLUS R²=0.9978, Nextera XT vs PLUS R²=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Back

R400750: SMART-Seq Single Cell PLUS Kit