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Advantage® 2 Polymerase Mix

Cat. #	Product	Size		License	Quantity	Details
639202	Advantage® 2 Polymerase Mix	5 x 100 Rxns	USD $1229.00
Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 500 PCR reactions of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639202: Advantage 2 Polymerase Mix

10X Advantage® 2 PCR Buffer

Cat. #	Product	Size		License	Quantity	Details
639137	10X Advantage® 2 PCR Buffer	2 x 600 uL	USD $95.00
This 10X PCR Buffer is designed for use with the Advantage 2 PCR Kit (Cat. # 639206 or 639207) and Advantage 2 Polymerase Mix (Cat. # 639201 or 639202). Suffi cient buffer is supplied for 240 PCR reactions of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639137: 10X Advantage 2 PCR Buffer

10X Advantage® 2 PCR Buffer

Cat. #	Product	Size		License	Quantity	Details
639138	10X Advantage® 2 PCR Buffer	10 mL	USD $208.00
This 10X PCR Buffer is designed for use with the Advantage 2 PCR Kit (Cat. # 639206 or 639207) and Advantage 2 Polymerase Mix (Cat. # 639201 or 639202). Sufficient buffer is supplied for 2,000 PCR reactions of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639138: 10X Advantage 2 PCR Buffer

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Notice to purchaser

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639202: Advantage 2 Polymerase Mix

Notice to purchaser

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639137: 10X Advantage 2 PCR Buffer

Notice to purchaser

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639138: 10X Advantage 2 PCR Buffer

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