Search results

Refine Your Search

638949

Search Filters

Product family

Cloning [1]

Application

Search results for

Available results will be divided among three tabs below.
Options for narrowing the number of matches are available on the left.

Adding terms to the "Refine Your Search" box will apply more criteria to your current results. Making selections in the "Search Filters" box will further limit your results within a variety of categories, such as application, product type, kit size, etc.

Showing 1-1 of 1 results

In-Fusion® Snap Assembly Master Mix

Cat. #	Product	Size		License	Quantity	Details
638949	In-Fusion® Snap Assembly Master Mix	250 Rxns	USD $2836.00
In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi. Back 638949: In-Fusion Snap Assembly Master Mix Back The In-Fusion cloning protocol The In-Fusion cloning protocol.