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Clontech® PCR-Select™ cDNA Subtraction Kit

Cat. # Product Size Price License Quantity Details
637401 Clontech® PCR-Select™ cDNA Subtraction Kit 7 Rxns USD $1057.00

Kit for identifying cDNAs that correspond to differentially expressed sequences in one cDNA population compared with another. Enough reagents are provided for seven cDNA reactions. PCR primers are provided for 50 primary and 100 secondary PCR amplifications—enough for one control and six complete subtractions if the cDNA from each synthesis is used for tester and driver in separate subtractions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
639206 Advantage® 2 PCR Kit 100 Rxns USD $459.00

This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 100 PCR reactions of 50 μl each.

639201 Advantage® 2 Polymerase Mix 100 Rxns USD $265.00

Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 μl each.

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639250 TaqStart® Antibody 200 Rxns USD $223.00

A monoclonal antibody (isotype IgG2b) that specifically binds to and inactivates Taq DNA polymerase below 70°C for use in hot-start PCR. PCR with TaqStart Antibody has been proven to prevent generation of nonspecific amplification products and primer-dimer artifacts.

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