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Stellar™ Competent Cells (dam-/dcm-)

Cat. # Product Size License Quantity Details
636764 Stellar™ Competent Cells (dam-/dcm-) 10 Transformations USD $222.00

Stellar Competent Cells (dam-/dcm-) are an E. coli HST04 strain that lacks the genetic factors (dam and dcm) necessary for the methylation of DNA. Plasmids prepared using this product can be cut by restriction enzymes which are normally blocked by dam or dcm methylation. A dam/recA double mutation is lethal, so the parent strain is recA+. Therefore, it follows that transformation of extracellular DNA with repeat sequences can result in recombination by recA. This product is not a suitable cloning host. For transformation, it is highly recommended to use this product for constructing plasmids as mentioned above. A pUC19 vector is provided as well as SOC Medium.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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