xTractor Buffer is an optimized buffer system for extraction of proteins from fresh or frozen bacterial cells, mammalian cells, yeast cells, or baculovirus-infected cells. This buffer is useful in extracting proteins over a broad range of molecular weights, including high molecular weight proteins that cannot be extracted unless the membrane is fully disrupted. Lysozyme and DNase I are included to help disrupt membranes and to reduce solution viscosity, respectively. xTractor Buffer is compatible with all resins, allowing quick purification of his-tagged proteins. Sufficient buffer is provided for 10 extractions of 1 g cell pellets.

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xTractor Cell Lysis Buffer simplifies extraction of his-tagged proteins using TALON resin

xTractor Cell Lysis Buffer simplifies extraction of his-tagged proteins using TALON resin. After extraction, you can purify your protein with either a standard TALON Resin column after centrifugation or filtration (left), or with the TALON CellThru Column (right).

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635623: xTractor Buffer Kit