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SMARTer® Human TCR a/b Profiling Kit

Cat. # Product Size Price License Quantity Details
635015 SMARTer® Human TCR a/b Profiling Kit 48 Rxns USD $3072.00

The SMARTer Human TCR a/b Profiling Kit enables users to analyze T-cell receptor (TCR) diversity from input RNA samples. This kit is designed to work with a range of RNA input amounts depending on the sample type, and has been shown to generate high-quality libraries from as little as 10 ng to 3 μg of total RNA obtained from peripheral blood leukocytes, or from 50 to 10,000 purified T cells. As the name suggests, the kit can be used to generate data for both alpha- and beta-chain diversity, either in the same experiment or separately.

This kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR transcripts. Included in the kit are primers that incorporate Illumina-specific adaptor sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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