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SMARTer® Stranded RNA-Seq Kit HT

Cat. # Product Size License Quantity Details
634862 SMARTer® Stranded RNA-Seq Kit HT 96 Rxns USD $4192.00

The SMARTer Stranded RNA-Seq Kit HT includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform in a high-throughput manner. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the Indexing Primer Set HT for Illumina (which contains 8 indexed forward PCR primers and 12 indexed reverse PCR primers for the high-throughput amplification of 96 uniquely-indexed RNA-seq libraries). This kit generates indexed, paired-end, Illumina-compatible sequencing libraries in a 96-well plate format, which enables high levels of multiplexing of NGS library analysis.

The SMARTer Stranded RNA-Seq Kit HT utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic cleanup or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. The Indexing Primer Set HT for Illumina integrated into this kit makes it convenient to generate 96 uniquely-indexed libraries for Illumina sequencing.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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SMARTer Stranded RNA-Seq Kits—strand-specific library construction for transcriptome analysis on Illumina platforms

The SMARTer Stranded RNA-Seq Kit generates indexed, Illumina-ready libraries with 99% accurate strand-of-origin data, in less than 4 hours.

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Unique Dual Index Kits

Unique Dual Index Kits are required for generating indexed Illumina-compatible NGS libraries for RNA-seq and DNA-seq. These products enable users to index and multiplex libraries of up to 384 samples.

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