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SMARTer® RACE 5’/3’ Kit

Cat. # Product Size Price License Quantity Details
634858 SMARTer® RACE 5’/3’ Kit 10 Rxns USD $691.00

The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.

RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5'/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene-specific RACE primers are supplied by the user.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Universal Primer Mix

Cat. # Product Size Price License Quantity Details
634922 Universal Primer Mix 100 Rxns USD $133.00

The Universal Primer Mix (UPM) adds suppression PCR elements to the ends of cDNA generated by our SMARTer RACE 5'/3' Kit (Cat. Nos. 634858 & 634859). The UPM consists of two primers: a long, 45-base primer and a short, 22-base primer. The Long Universal Primer (Long UP) is composed of two parts: the 23 bases at the 3' end of the primer are identical to those at the 5' end of the SMARTer IIA Oligo; the 22 bases at the 5' end of the primer provide the suppression sequence for suppression PCR. The Short Universal Primer (Short UP) sequence is identical to that of the 22 bases at the 5' end of the Long UP. The Short UP, for which the binding site is introduced by the Long UP during the first round of PCR, is present in the UPM at 5 times the concentration of the Long UP.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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cDNA amplification kits for 5' and 3' RACE

Obtain full-length sequences of RNA transcripts found within a cell, using a highly sensitive method powered by SMART technology.

Showing 1-3 of 3 results