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Next generation sequencing [1]

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mRNA-seq [1]

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SMARTer® Ultra® Low Input RNA for Illumina® Sequencing - HV

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Product

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Details

634828

SMARTer® Ultra® Low Input RNA for Illumina® Sequencing - HV

96 Rxns

USD $8192.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzersequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase