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SMARTer® Human TCR a/b Profiling Kit v2

Cat. #	Product	Size		License	Quantity	Details
634479	SMARTer® Human TCR a/b Profiling Kit v2	48 Rxns	USD $3850.00
The SMARTer Human TCR a/b Profiling Kit v2 enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 100 ng T cells) or purified T cells (1,000 to 10,000 cells) and can be used to generate data for both TCR-alpha or TCR-beta subunits. Unique molecular identifiers (UMIs) are incorporated to facilitate PCR error correction and clonotype quantification during data analysis, and unique dual indexes (UDIs) allow users to multiplex more samples and to provide confidence when sequencing on patterned flow cells. At the end, generated indexed libraries are ready for sequencing on Illumina platforms. This kit supports up to 48 rxns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts. Variable and highly complex samples are accommodated without the need for RNA purification or T-cell enrichment. TRA and TRB libraries were generated from 1, 10, and 100 ng of human CD3+ T‑cell total RNA (Panel A), 1,000 and 10,000 CD3+ T cells (Panel B), and 20 ng of whole-blood RNA extracted from three different samples (Panel C). The sequence reads were processed by CogentIP. Back The Takara Bio TCR profiling kit identifies a wide range of clonotype counts. The number of clonotypes detected reveals biological variation. Duplicate libraries were generated from 10 ng RNA extracted from single-donor PBMC samples (P1-P6) and sequenced on an Illumina MiSeq system using 300-bp paired-end reads to obtain approximately 1.5 million reads per sample. Resulting sequencing reads were processed with CogentIP. Clonotype numbers from different donors for TRA (blue) and TRB (orange) are shown. Error bars shown represent the standard error between the duplicates. Back The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors A dramatically higher clonotype number was observed for TRB after downsampling. (TRA results were similar; data not shown.) 5M PBMC cells were split from two different healthy donors for RNA and gDNA extraction. 1.6 g of gDNA (15% of the total amount of extracted gDNA) was used for library preparation according to manufacturer's instructions. 100 ng of RNA (2% of the total amount of extracted RNA) was used for library preparation. Back 634479: SMARTer Human TCR a/b Profiling Kit v2

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Details

634479

SMARTer® Human TCR a/b Profiling Kit v2

48 Rxns

USD $3850.00

The SMARTer Human TCR a/b Profiling Kit v2 enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 100 ng T cells) or purified T cells (1,000 to 10,000 cells) and can be used to generate data for both TCR-alpha or TCR-beta subunits. Unique molecular identifiers (UMIs) are incorporated to facilitate PCR error correction and clonotype quantification during data analysis, and unique dual indexes (UDIs) allow users to multiplex more samples and to provide confidence when sequencing on patterned flow cells. At the end, generated indexed libraries are ready for sequencing on Illumina platforms.

This kit supports up to 48 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Unique Dual Index Kits

Unique Dual Index Kits are required for generating indexed Illumina-compatible NGS libraries for RNA-seq and DNA-seq. These products enable users to index and multiplex libraries of up to 384 samples.

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SMARTer Human TCR a/b Profiling Kit v2—flexible kit with ultimate sensitivity for understanding the TCR repertoire

SMARTer TCR profiling with optimized chemistry, UMIs, and UDIs for more accurate, reliable clonotype calling and quantification.

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Notice to purchaser

Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts.

The Takara Bio TCR profiling kit identifies a wide range of clonotype counts.

The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors

634479: SMARTer Human TCR a/b Profiling Kit v2

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