The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of BCR transcripts. It also incorporates unique molecular identifiers (UMI) to facilitate PCR error correction and clonotype quantification during data analysis.

Included in the kit are primers that incorporate Illumina-specific adapter sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms. This kit supports up to 48 rxns.

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634467: SMARTer Human BCR IgG IgM H/K/L Profiling Kit