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Guide-it™ Genotype Confirmation Kit

Cat. # Product Size Price License Quantity Details
632611 Guide-it™ Genotype Confirmation Kit 100 Rxns USD $459.00

The CRISPR/Cas9 system has been harnessed to create a simple, RNA-programmable method to mediate genome editing in mammalian cells. CRISPR/Cas9 editing generates insertions or deletions (indels) that can result in gene knockout. In most cases, cells have two copies of any given gene and indel mutations can be generated in either one or both alleles. The Guide-it Genotype Confirmation Kit provides a simple protocol to determine whether gene editing resulted in indels on one allele (monoallelic) or both alleles (biallelic) in singly isolated cells (clones), allowing for the identification of clones with desired mutations for further analysis. The kit is designed to be used in conjunction with the Guide-it sgRNA In Vitro Transcription Kit (Cat. No. 632635), which generates a target-specific guide RNA used in the genotype confirmation reaction. The sequence of the guide RNA used for the genotype confirmation reaction is identical to the guide RNA used for the initial genome editing experiment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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